Artykuł w czasopiśmie
Brak miniatury
Licencja
ClosedAccessDostęp zamknięty
 

Genome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp. DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors

Uproszczony widok
dc.abstract.enPsychrobacter sp. DAB_AL43B, isolated from ornithogenic soil collected on the Arctic island of Spitsbergen, is a newly sequenced psychrophilic strain susceptible to conjugation and electrotransformation. Its genome consists of a circular chromosome (3.3 Mb) and four plasmids (4.4-6.4kb). In silico genome mining and microarray-based phenotypic analysis were performed to describe the metabolic potential of this strain and identify possible biotechnological applications. Metabolic reconstruction indicated that DAB_AL43B prefers low-molecular-weight carboxylates and amino acids as carbon and energy sources. Genetic determinants of heavy-metal resistance, anthracene degradation and possible aerobic denitrification were also identified. Comparative analyses revealed a relatively close relationship between DAB_AL43B and other sequenced Psychrobacter species. In addition, the plasmids of this strain were used as the basis for the construction of Escherichia coli-Psychrobacter spp. shuttle vectors. Taken together, the results of this work suggest that DAB_AL43B is a promising candidate as a new model strain for studies on Psychrobacter spp.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorWibberg, Daniel
dc.contributor.authorJędrys, Zuzanna
dc.contributor.authorPuhler, Alfred
dc.contributor.authorSchluter, Andreas
dc.contributor.authorRomaniuk, Krzysztof
dc.contributor.authorBartosik, Dariusz
dc.contributor.authorSzych, Anna
dc.contributor.authorLasek, Robert
dc.contributor.authorDecewicz, Przemysław
dc.date.accessioned2024-01-25T02:05:35Z
dc.date.available2024-01-25T02:05:35Z
dc.date.issued2017
dc.description.financeNie dotyczy
dc.description.volume263
dc.identifier.doi10.1016/J.JBIOTEC.2017.09.011
dc.identifier.issn0168-1656
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/107843
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/abs/pii/S0168165617316516?via%3Dihub
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofJournal of Biotechnology
dc.relation.pages64-74
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enComparative genomics
dc.subject.enComplete genome
dc.subject.enMetabolic reconstruction
dc.subject.enPhenotypic microarrays
dc.subject.enPsychrobacter sp
dc.subject.enPsychrophile
dc.titleGenome content, metabolic pathways and biotechnological potential of the psychrophilic Arctic bacterium Psychrobacter sp. DAB_AL43B, a source and a host of novel Psychrobacter-specific vectors
dc.typeJournalArticle
dspace.entity.typePublication