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Ciprofloxacin and nalidixic acid resistance of Salmonella spp. isolated from retail food in Poland

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dc.abstract.enDistribution of aminoacid substitutions in the quinolone resistance-determining region (QRDRs) of gyrA, gyrB, parC, parE and determinants of plasmid-mediated quinolone resistance (PMQR) were investigated among resistant to quinolones Salmonella spp. strains isolated from retail food in Poland in years 2008-2013. Ten different aminoacid substitutions were identified in QRDRs. Five different aminoacid substitutions were identified in gyrA: Ser83Tyr, Ser83Phe, Asp87Tyr, Asp87Asn, Asp87Gly, two aminoacid substitutions both in parC: Thr57Ser, Ser80Ile and in parE: Leu445Phe, Arg511Ser. One substitution - Ser464Phe - was detected within gyrB. Single substitution in gyrA (Ser83Tyr) were identified the most frequently – 34.8% (63/181). Second, the most frequently identified variant (21.0% - 38/181) were co-existence of two single substitutions in gyrA: Ser83Tyr and parC: Thr57Ser. In four isolates co-existed three substitutions in three different genes gyrA: Ser83Tyr + parC: Thr57Ser + parE: Leu445Phe (two isolates), gyrA: Ser83Phe + parC: Thr57Ser + parE: Leu445Phe, gyrA: Ser83Tyr + parC: Thr57Ser + parE: Arg511Ser. In two isolates, four substitutions were identified - in gyrA: Ser83Phe + Asp87Tyr and in parC: Thr57Ser + Ser80Ile. Among resistant isiolates, MIC values varied between 32 and 2048 mg/L (nalidixic acid) and between 0,125 – 16 mg/L (ciprofloxacin). MIC values of two isolates harboring qnrS1without any substitutions were 32 mg/L (NA) and 0,5-1,0 mg/L (CIP). The highest MIC values for NA and CIP were observed in two isolates of Salmonella spp. carrying double substitutions in gyrA: Ser83Phe + Asp87Tyr and parC: Thr57Ser + Ser80Ile. MIC value for NA was 2048 mg/L while for CIP – 16 mg/L.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWołkowicz, Tomasz
dc.contributor.authorMaćkiw, Elżbieta
dc.contributor.authorKowalska, Joanna
dc.contributor.authorPopowska, Magdalena
dc.contributor.authorMąka, Łukasz
dc.contributor.authorPostupolski, Jacek
dc.contributor.authorStasiak, Monika
dc.date.accessioned2024-01-24T19:24:39Z
dc.date.available2024-01-24T19:24:39Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.volume276
dc.identifier.doi10.1016/J.IJFOODMICRO.2018.03.012
dc.identifier.issn0168-1605
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/103084
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0168160518300886?via%3Dihub
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Food Microbiology
dc.relation.pages1-4
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enSalmonella spp. quinolones QRDR mutations PMQR antimicrobial resistance food
dc.titleCiprofloxacin and nalidixic acid resistance of Salmonella spp. isolated from retail food in Poland
dc.typeJournalArticle
dspace.entity.typePublication