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Dostęp zamkniętyEfficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number
| dc.abstract.en | Growth of human nonadherent HL-60 cell cultures performed in disposable bioreactor under various hydrodynamic conditions of 2-D wave-assisted agitation has been compared and discussed. Influence of Reynolds number for liquid (ReL) and thekLacoefficient, as key parameters characterized the bioprocessing of HL-60 cells inReadyToProcessWAVETM25 system, on reached values of the apparent maximal specific growth rate (μmax) and the specific yield of biomass (Y*X/S) has been identified. The values of ReL(i.e., 510–10,208), as well askLacoefficient (i.e., 2.83–13.55 h−1), have been estimated for the cultures subjected to wave-induced mixing, based on simplified dimensionless correlation for various presents of WAVE 25 system. The highest values of apparentμmax = 0.038 h−1andY*X/S = 25.64 × 108cells gglc−1have been noted for cultures independently performed at wave-induced agitation characterized by ReLequaled to 5104 and 510, respectively. The presented results have high applicability potential in scale-up of bioprocesses focused on nonadherent animal cells, or in the case of any application of disposable bioreactors presenting similitude. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Wierzchowski, Kamil |
| dc.contributor.author | Pilarek, Maciej |
| dc.contributor.author | Grabowska-Kowalik, Iwona |
| dc.date.accessioned | 2024-01-24T22:31:43Z |
| dc.date.available | 2024-01-24T22:31:43Z |
| dc.date.issued | 2020 |
| dc.description.finance | Publikacja bezkosztowa |
| dc.description.volume | 43 |
| dc.identifier.doi | 10.1007/S00449-020-02386-6 |
| dc.identifier.issn | 1615-7591 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/105875 |
| dc.identifier.weblink | https://link.springer.com/article/10.1007/s00449-020-02386-6 |
| dc.language | eng |
| dc.pbn.affiliation | biological sciences |
| dc.relation.ispartof | Bioprocess and Biosystems Engineering |
| dc.relation.pages | 1973-1985 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | disposable (single-use) bioreactor |
| dc.subject.en | wave-type agitation |
| dc.subject.en | Re number |
| dc.subject.en | kLa coefficient |
| dc.subject.en | human hematopoietic HL-60 cells |
| dc.subject.en | nonadherent cell propagation |
| dc.title | Efficient propagation of suspended HL-60 cells in a disposable bioreactor supporting wave-induced agitation at various Reynolds number |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |