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Benefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B

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cris.lastimport.scopus2024-02-12T20:01:16Z
dc.abstract.enPsychrobacter sp. DAB_AL32B, originating from Spitsbergen island (Arctic), carries the large plasmid pP32BP2 (54,438 bp). Analysis of the pP32BP2 nucleotide sequence revealed the presence of three predicted phenotypic modules that comprise nearly 30% of the plasmid genome. These modules appear to be involved in fimbriae synthesis via the chaperone-usher pathway (FIM module) and the aerobic and anaerobic metabolism of carnitine (CAR and CAI modules, respectively). The FIM module was found to be functional in diverse hosts since it facilitated the attachment of bacterial cells to abiotic surfaces, enhancing biofilm formation. The CAI module did not show measurable activity in any of the tested strains. Interestingly, the CAR module enabled the enzymatic breakdown of carnitine, but this led to the formation of the toxic by-product trimethylamine, which inhibited bacterial growth. Thus, on the one hand, pP32BP2 can enhance biofilm formation, a highly advantageous feature in cold environments, while on the other, it may prevent bacterial growth under certain environmental conditions. The detrimental effect of harboring pP32BP2 (and its CAR module) seems to be conditional, since this replicon may also confer the ability to use carnitine as an alternative carbon source, although a pathway to utilize trimethylamine is most probably necessary to make this beneficial. Therefore, the phenotype determined by this CAR-containing plasmid depends on the metabolic background of the host strain.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDziewit, Łukasz
dc.contributor.authorBartosik, Dariusz
dc.contributor.authorCegielski, Adrian
dc.contributor.authorCiok, Anna
dc.date.accessioned2024-01-24T18:34:23Z
dc.date.available2024-01-24T18:34:23Z
dc.date.copyright2019-04-24
dc.date.issued2019
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.identifier.doi10.3390/IJMS20082015
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102113
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enArctic
dc.subject.enPsychrobacter
dc.subject.enadhesion
dc.subject.enbiofilm formation
dc.subject.encarnitine metabolism
dc.subject.enplasmid
dc.subject.enpsychrophile
dc.subject.entrimethylamine
dc.titleBenefits and Drawbacks of Harboring Plasmid pP32BP2, Identified in Arctic Psychrophilic Bacterium Psychrobacter sp. DAB_AL32B
dc.typeJournalArticle
dspace.entity.typePublication