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Dostęp zamkniętySynthesis of the first double-functionalized dinucleotide mRNA cap analogue for its specific labeling
| cris.lastimport.scopus | 2024-02-12T19:29:52Z |
| dc.abstract.en | The modification of various important nucleotide-based molecules (such as nucleotides, RNA, DNA, oligonucleotides) with fluorophores, affinity tags and reactive moieties is of enormous utility for studying their localization, structure and dynamics, as well as diverse biological functions involving their interacting partners. Herein, we report chemical methodology in which the dinucleotide mRNA cap analogue is doubly modified within its second nucleotide. The prepared dinucleotide contains an alkyne at the N2-position of guanine, and levulinic acid within the ribose moiety. Such modifications may be further used for specific labeling of the cap, for instance with a fluorophore that will allow the molecule to be tracked inside the cell and an attachment cell-penetrating peptide that will help to deliver it to the area of interest. Exemplar molecules were attached in order to demonstrate the utility of the newly synthesized cap analogue. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Kałużna, Justyna |
| dc.contributor.author | Kryńska, Paulina |
| dc.contributor.author | Piecyk, Karolina |
| dc.contributor.author | Jankowska-Anyszka, Marzena |
| dc.date.accessioned | 2024-01-26T09:26:59Z |
| dc.date.available | 2024-01-26T09:26:59Z |
| dc.date.issued | 2017 |
| dc.description.finance | Nie dotyczy |
| dc.description.volume | 58 |
| dc.identifier.doi | 10.1016/J.TETLET.2017.06.060 |
| dc.identifier.issn | 0040-4039 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/121320 |
| dc.language | eng |
| dc.pbn.affiliation | chemical sciences |
| dc.relation.ispartof | Tetrahedron Letters |
| dc.relation.pages | 3037-3040 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | Cap analogues |
| dc.subject.en | Dinucleotide |
| dc.subject.en | 5′ mRNA cap |
| dc.subject.en | Molecular probe |
| dc.subject.en | Labelling |
| dc.title | Synthesis of the first double-functionalized dinucleotide mRNA cap analogue for its specific labeling |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |