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Molecular mechanisms of photoadaptation of photosystem I supercomplex from an evolutionary cyanobacterial/algal intermediate

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dc.abstract.en<p>The monomeric photosystem I-light-harvesting antenna complex I (PSI-LHCI) supercomplex from the extremophilic red alga Cyanidioschyzon merolae represents an intermediate evolutionary link between the cyanobacterial PSI reaction center and its green algal/higher plant counterpart. We show that the C. merolae PSI-LHCI supercomplex is characterized by robustness in various extreme conditions. By a combination of biochemical, spectroscopic, mass spectrometry, and electron microscopy/single particle analyses, we dissected three molecular mechanisms underlying the inherent robustness of the C. merolae PSI-LHCI supercomplex: (1) the accumulation of photoprotective zeaxanthin in the LHCI antenna and the PSI reaction center; (2) structural remodeling of the LHCI antenna and adjustment of the effective absorption cross section; and (3) dynamic readjustment of the stoichiometry of the two PSI-LHCI isomers and changes in the oligomeric state of the PSILHCI supercomplex, accompanied by dissociation of the PsaK core subunit. We show that the largest low light-treated C. merolae PSI-LHCI supercomplex can bind up to eight Lhcr antenna subunits, which are organized as two rows on the PsaF/ PsaJ side of the core complex. Under our experimental conditions, we found no evidence of functional coupling of the phycobilisomes with the PSI-LHCI supercomplex purified from various light conditions, suggesting that the putative association of this antenna with the PSI supercomplex is absent or may be lost during the purification procedure.</p>
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKargul, Joanna
dc.contributor.authorHaniewicz, Patrycja
dc.contributor.authorNosek, Lukas
dc.contributor.authorKirkpatrick, Joanna
dc.contributor.authorEl-Mohsnawy, Eithar
dc.contributor.authorKouril, Roman
dc.contributor.authorAbram, Mateusz
dc.contributor.authorOlmos, Julian Janna
dc.date.accessioned2024-01-25T12:53:22Z
dc.date.available2024-01-25T12:53:22Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.volume176
dc.identifier.doi10.1104/PP.17.01022
dc.identifier.issn0032-0889
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/112866
dc.identifier.weblinkhttp://www.plantphysiol.org/content/176/2/1433
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofPlant Physiology
dc.relation.pages1433-1451
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleMolecular mechanisms of photoadaptation of photosystem I supercomplex from an evolutionary cyanobacterial/algal intermediate
dc.typeJournalArticle
dspace.entity.typePublication