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Ultrathin glass fiber microprobe for electroporation of arbitrary selected cell groups

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dc.abstract.enA new type of ultrathin fiber microprobe for selective electroporation is reported. The microprobe is 10 cm long and has a diameter of 350 µm. This microprobe is a low cost tool, which allows electroporation of an arbitrary selected single cell or groups of cells among population with use of a standard microscope and cell culture plates. The microprobe in its basic form contains two metal microelectrodes made of a silver-copper alloy, running along the fiber, each with a diameter of 23 µm. The probe was tested in vitro on a population of normal and cancer cells. Successful targeted electroporation was observed by means of accumulation of trypan blue (TB) dye marker in the cell. The electroporation phenomenon was also verified with propidium iodide and Annexin V in fluorescent microscopy.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKotulska, Małgorzata
dc.contributor.authorPysz, Dariusz
dc.contributor.authorSaczko, Jolanta
dc.contributor.authorMiklavčič, Damijan
dc.contributor.authorKulbacka, Julita
dc.contributor.authorStępień, Ryszard
dc.contributor.authorStępniewski, Grzegorz
dc.contributor.authorBuczyński, Ryszard
dc.contributor.authorKasztelanic, Rafał
dc.date.accessioned2024-01-26T11:18:25Z
dc.date.available2024-01-26T11:18:25Z
dc.date.issued2020
dc.description.financePublikacja bezkosztowa
dc.description.volume135
dc.identifier.doi10.1016/J.BIOELECHEM.2020.107545
dc.identifier.issn1567-5394
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/124109
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S1567539419307303?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationphysical sciences
dc.relation.ispartofBioelectrochemistry
dc.relation.pages107545
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enElectroporation
dc.subject.enOptical fibers
dc.subject.enElectrochemotherapy
dc.subject.enMicroprobe
dc.subject.enDrug delivery
dc.titleUltrathin glass fiber microprobe for electroporation of arbitrary selected cell groups
dc.typeJournalArticle
dspace.entity.typePublication