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Fullerenol C60(OH)36 protects human erythrocyte membrane against high-energy electrons

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cris.lastimport.scopus2024-02-12T19:53:38Z
dc.abstract.enFullerenols (polyhydroxylated fullerene C60) are nanomaterial with potentially broad applicability in biomedical sciences with high antioxidant ability, thus, we investigated the radioprotecting potential of fullerenol C60(OH)36 on human erythrocytes irradiated by high-energy electrons of 6 MeV. The results demonstrate that C60(OH)36 at concentration of 150 μg/mL protects the erythrocytes against the radiation-induced hemolysis (comparing to non-protected cells, we observed 30% and 39% protection for 0.65 and 1.3 kGy irradiation doses, respectively). The protecting effect was confirmed by 32% decreased release of potassium cations comparing to the cells irradiated without C60(OH)36. Measurements of the amount of lactate dehydrogenase (LDH) released from the irradiated erythrocytes showed that the size of the pores formed by irradiation was not sufficient to release LDH across the erythrocyte membranes. We also report a significant decrease of the affinity of acetylcholinesterase (AChE) for the substrate in the presence of fullerenol, indicating the relatively strong adsorption of C60(OH)36 to components of plasma membrane. Changes in membrane fluidity detected by fluorescence spectroscopy and conformational changes in membrane proteins detected by spin labeling suggest the dose-dependent formation of disulfide groups as an effect of oxidation and this process was inhibited by C60(OH)36. We suppose that scavenging the ROS as well as adsorption of fullerenol to membrane proteins and steric protection of -SH groups against oxidation are responsible for the observed effects.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWolszczak, Marian
dc.contributor.authorKrokosz, Anita
dc.contributor.authorKaźmierska-Grębowska, Paulina
dc.contributor.authorGrębowski, Jacek
dc.contributor.authorLitwinienko, Grzegorz
dc.contributor.authorLankoff, Anna
dc.date.accessioned2024-01-25T01:37:49Z
dc.date.available2024-01-25T01:37:49Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number8
dc.description.volume1860
dc.identifier.doi10.1016/J.BBAMEM.2018.05.005
dc.identifier.issn0005-2736
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/107597
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0005273618301470
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofBiochimica et Biophysica Acta - Biomembranes
dc.relation.pages1528-1536
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enFullerenol
dc.subject.enMembrane fluidity
dc.subject.enMembrane damage
dc.subject.enThiol groups
dc.subject.enHemolysis
dc.subject.enRadioprotection
dc.titleFullerenol C60(OH)36 protects human erythrocyte membrane against high-energy electrons
dc.typeJournalArticle
dspace.entity.typePublication