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Novel electrogravimetric biosensors for the ultrasensitive detection of plasma matrix metalloproteinase-2 considered a potential tumor biomarker

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cris.lastimport.scopus2024-02-12T19:53:41Z
dc.abstract.enIn this study, we developed novel, simple gravimetric and voltammetric sensors for the ultrasensitive detection of active matrix metalloproteinase (MMP)-2 in plasma. The developed sensors are cost-effective, require a very less amount of reagents, and are time-saving. They detect MMP-2 based on antigen–antibody recognition and its ability to cleave glycine-leucine peptide bond. The three-dimensional bioplatform of the sensors consisted of a cationic polyethyleneimine (PEI) polymer that facilitated robust immobilization of the dipeptide labeled with anthraquinone (AQ), or antibody molecules in appropriate density, which was crucial for biosensing. Detection was performed using quartz crystal microbalance with dissipation and voltammetry. The results showed that the developed sensors were characterized by high stability, wide analytical range (2.0 pg mL−1 to 5.0 μg mL−1), and low detection limit (ca. 10 fg mL−1). They also exhibited excellent efficiency in the determination of active MMP-2 in real samples, such as blood plasma. The developed sensors may hold great promise for the early diagnosis of cancers.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorNowicka, Anna
dc.contributor.authorKowalczyk, Agata
dc.contributor.authorGrudziński, Ireneusz
dc.contributor.authorBamburowicz-Klimkowska, Magdalena
dc.contributor.authorCupriak, Aleksandra
dc.contributor.authorGajda, Aleksandra
dc.contributor.authorNisiewicz, Monika
dc.date.accessioned2024-01-25T13:52:19Z
dc.date.available2024-01-25T13:52:19Z
dc.date.copyright2021-11-18
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.versionFINAL_PUBLISHED
dc.description.volume1191
dc.identifier.doi10.1016/J.ACA.2021.339290
dc.identifier.issn0003-2670
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113953
dc.identifier.weblinkhttp://www.journals.elsevier.com/analytica-chimica-acta/
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofAnalytica Chimica Acta
dc.relation.pages339290
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enMetalloproteinase-2
dc.subject.enActive form
dc.subject.enProtease
dc.subject.enImmunosensor
dc.subject.enCleavage of peptide bond
dc.subject.enElectrogravimetry
dc.titleNovel electrogravimetric biosensors for the ultrasensitive detection of plasma matrix metalloproteinase-2 considered a potential tumor biomarker
dc.typeJournalArticle
dspace.entity.typePublication