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Heterodimerizing helices as tools for nanoscale control of the organization of protein-protein and protein-quantum dots

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cris.lastimport.scopus2024-02-12T19:59:25Z
dc.abstract.enIn this study, we tested the possibility of creating complexes of two proteins by fusing them with heterodimerizing helices. We used the fluorescent proteins GFP and mCHERRY expressed with a His-tag as our model system. We added heterodimer-forming sequences at the C- or N- termini of the proteins, opposite to the His-tag position. Heterodimerization was tested for both helices at the C-terminus or at the N- terminus and C-terminus. We observed complex formation with a nanomolar dissociation constant in both cases that was higher by one order of magnitude than the Kds measured for helices alone. The binding of two C-terminal helices was accompanied by an increased enthalpy change. The binding between helices could be stabilized by introducing an additional turn of the helix with cysteine, which was capable of forming disulphide bridges. Covalently linked proteins were obtained using this strategy and observed using fluorescence cross-correlation spectroscopy. Finally, we demonstrated the formation of complexes of protein dimers and quantum dots.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorJemioła-Rzemińska, Małgorzata
dc.contributor.authorSztatelman, Olga
dc.contributor.authorKopeć, Katarzyna
dc.contributor.authorPędziwiatr, Marta
dc.contributor.authorTrojan, Martyna
dc.contributor.authorWorch, Remigiusz
dc.contributor.authorWielgus-Kutrowska, Beata
dc.contributor.authorGrzyb, Joanna
dc.contributor.authorBzowska, Maria
dc.date.accessioned2024-01-25T03:07:52Z
dc.date.available2024-01-25T03:07:52Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.volume167
dc.identifier.doi10.1016/J.BIOCHI.2019.09.015
dc.identifier.issn0300-9084
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108405
dc.languageeng
dc.pbn.affiliationphysical sciences
dc.relation.ispartofBiochimie
dc.relation.pages93-105
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enheterodimerizing helices
dc.subject.enquantum dots
dc.subject.ennanohybrids
dc.titleHeterodimerizing helices as tools for nanoscale control of the organization of protein-protein and protein-quantum dots
dc.typeJournalArticle
dspace.entity.typePublication