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Polyelectrolyte membrane scaffold sustains growth of neuronal cells

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dc.abstract.enCell immobilization within nano-thin polymeric shells can provide an optimal concentration of biological material in a defined space and facilitate its directional growth. Herein, polyelectrolyte membrane scaffolds were constructed using a layer-by-layer approach to determine the possibility of promoting improved growth of rat cortical neuronal cells. Membrane presence was confirmed by Fourier transform infrared spectroscopy, Zeta potential, and atomic force and scanning electron microscopy. Scaffold performance toward neuronal cell growth was assessed in vitro during a 14-day culture. Cell conditions were analyzed immunocytochemically. Furthermore, western blot and real-time PCR analyses were used to validate the presence of neuronal and glial cells on the scaffolds. We observed that alginate/chitosan, alginate/polylysine, and polyethyleneimine/chitosan scaffolds promote neuronal growth similarly to the control, poly-d-lysine/laminin. We conclude that membranes maintaining cell viability, integrity and immobilization in systems supporting neuronal regeneration can be applied in neurological disease or wound healing treatment. (c) 2018 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 839-850, 2019.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKuznicki, J.
dc.contributor.authorKoźbiał, Małgorzata
dc.contributor.authorGruszczynska-Biegala, J.
dc.contributor.authorCZEREDYS, MAGDALENA
dc.contributor.authorGRZECZKOWICZ, Anna
dc.contributor.authorKwiatkowska, Angelika
dc.contributor.authorGranicka, Ludomira
dc.contributor.authorStrawski, Marcin
dc.contributor.authorSzklarczyk, Marek
dc.date.accessioned2024-01-25T17:02:27Z
dc.date.available2024-01-25T17:02:27Z
dc.date.copyright2018-12-26
dc.date.issued2019
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number4
dc.description.versionFINAL_PUBLISHED
dc.description.volume107
dc.identifier.doi10.1002/JBM.A.36599
dc.identifier.issn1549-3296
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/116464
dc.identifier.weblinkhttps://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36599
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofJournal of Biomedical Materials Research - Part A
dc.relation.pages839-850
dc.rightsCC-BY-NC
dc.sciencecloudnosend
dc.subject.enimmobilization
dc.subject.enpolyelectrolyte
dc.subject.enneuronal and glial cells
dc.subject.enlayer-by-layer
dc.titlePolyelectrolyte membrane scaffold sustains growth of neuronal cells
dc.typeJournalArticle
dspace.entity.typePublication