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Elimination of 01/A'-A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3

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dc.abstract.enPre-rRNA processing generates mature 18S, 5.8S, and 28S/25S rRNAs through multistage removal of surrounding 5'-ETS/3'-ETS and intervening ITS1/ITS2 segments. Endonucleolytic activities release by-products, which need to be eliminated. Here, we investigated the interplay of exosome-associated 3'-5' exonucleases DIS3 and RRP6 in rRNA processing and by-product elimination in human cells. In agreement with previous reports, we observed accumulation of 5.8S and 18S precursors upon dysfunction of these enzymes. However, none of these phenotypes was so pronounced as previously overlooked accumulation of short RNA species derived from 5'-ETS (01/A'-A0), in cells with nonfunctional DIS3. We demonstrate that removal of 01/A'-A0 is independent of the XRN2 5'-3' exonucleolytic activity. Instead, it proceeds rapidly after AO cleavage and occurs exclusively in the 3'-5' direction in several phases-following initiation by an unknown nuclease, the decay is executed by RRP6 with some contribution of DIS3, whereas the ultimate phase involves predominantly DIS3. Our data shed new light onto the role of human exosome in 5'-ETS removal. Furthermore, although 01/A'-AO degradation involves the action of two nucleases associated with the exosome ring, similarly to 5.8S 3'-end maturation, it is likely that contrary to the latter process, RRP6 acts prior to or redundantly with DIS3.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKobyłecki, Kamil
dc.contributor.authorTomecki, Rafał
dc.contributor.authorDziembowski, Andrzej
dc.contributor.authorKuliński, Tomasz
dc.contributor.authorDrążkowska, Karolina
dc.date.accessioned2024-01-24T22:46:29Z
dc.date.available2024-01-24T22:46:29Z
dc.date.issued2018
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number12
dc.description.versionFINAL_PUBLISHED
dc.description.volume24
dc.identifier.doi10.1261/RNA.066589.118
dc.identifier.issn1355-8382
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/106050
dc.identifier.weblinkhttps://rnajournal.cshlp.org/content/24/12/1677
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofRNA
dc.relation.pages1677-1692
dc.rightsCC-BY-NC
dc.sciencecloudnosend
dc.subject.enpre-rRNA processing
dc.subject.enexosome
dc.subject.enDI53
dc.subject.enRRP6
dc.subject.enXRN2
dc.titleElimination of 01/A'-A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3
dc.typeJournalArticle
dspace.entity.typePublication