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More P450s Are Involved in Secondary Metabolite Biosynthesis in Streptomyces Compared to Bacillus, Cyanobacteria, and Mycobacterium

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dc.abstract.enUnraveling the role of cytochrome P450 monooxygenases (CYPs/P450s), heme-thiolateproteins present in living and non-living entities, in secondary metabolite synthesis is gainingmomentum. In this direction, in this study, we analyzed the genomes of 203Streptomycesspeciesfor P450s and unraveled their association with secondary metabolism. Our analyses revealed thepresence of 5460 P450s, grouped into 253 families and 698 subfamilies. The CYP107 family wasfound to be conserved and highly populated inStreptomycesandBacillusspecies, indicating its keyrole in the synthesis of secondary metabolites.Streptomycesspecies had a higher number of P450sthanBacillusand cyanobacterial species. The average number of secondary metabolite biosyntheticgene clusters (BGCs) and the number of P450s located in BGCs were higher inStreptomycesspeciesthan inBacillus, mycobacterial, and cyanobacterial species, corroborating the superior capacity ofStreptomycesspecies for generating diverse secondary metabolites. Functional analysisviadata miningconfirmed that manyStreptomycesP450s are involved in the biosynthesis of secondary metabolites.This study was the first of its kind to conduct a comparative analysis of P450s in such a large number(203) ofStreptomycesspecies, revealing the P450s’ association with secondary metabolite synthesisinStreptomycesspecies. Future studies should include the selection ofStreptomycesspecies with ahigher number of P450s and BGCs and explore the biotechnological value of secondary metabolitesthey produce.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorSyed, Khajamohiddin
dc.contributor.authorNelson, David R.
dc.contributor.authorGront, Dominik
dc.contributor.authorPadayachee, Tiara
dc.contributor.authorMnguni, Fanele Cabangile
dc.contributor.authorYu, Jae-Hyuk
dc.contributor.authorChen, Wanping
dc.date.accessioned2024-01-25T12:55:20Z
dc.date.available2024-01-25T12:55:20Z
dc.date.copyright2020-07-07
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number13
dc.description.versionFINAL_PUBLISHED
dc.description.volume21
dc.identifier.doi10.3390/IJMS21134814
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/112955
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/21/13/4814/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages4814
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enBacillus
dc.subject.enBiosynthetic gene clusters
dc.subject.enCyanobacteria
dc.subject.enCytochrome P450 monooxygenases
dc.subject.enMycobacterium
dc.subject.enNon-ribosomal peptides
dc.subject.enP450 blooming
dc.subject.enPolyketides
dc.subject.enSecondary metabolites
dc.subject.enStreptomyces
dc.subject.enTerpenes
dc.titleMore P450s Are Involved in Secondary Metabolite Biosynthesis in Streptomyces Compared to Bacillus, Cyanobacteria, and Mycobacterium
dc.typeJournalArticle
dspace.entity.typePublication