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Bromodomain-containing subunits BRD1, BRD2, and BRD13 are required for proper functioning of SWI/SNF complexes in Arabidopsis

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dc.abstract.enSWI/SNF chromatin remodelers are evolutionarily conserved multiprotein complexes that use the energy of ATP hydrolysis to change chromatin structure. A characteristic feature of SWI/SNF remodelers is the occurrence in both the catalytic ATPase subunit and some auxiliary subunits, of bromodomains, the protein motifs capable of binding acetylated histones. Here, we report that the Arabidopsis bromodomain-containing proteins BRD1, BRD2, and BRD13 are likely true SWI/SNF subunits that interact with the core SWI/SNF components SWI3C and SWP73B. Loss of function of each single BRD protein caused early flowering but had a negligible effect on other developmental pathways. By contrast, a brd triple mutation (brdx3) led to more pronounced developmental abnormalities, indicating functional redundancy among the BRD proteins. The brdx3 phenotypes, including hypersensitivity to abscisic acid and the gibberellin biosynthesis inhibitor paclobutrazol, resembled those of SWI/SNF mutants. Furthermore, the BRM protein level and occupancy at the direct target loci SCL3, ABI5, and SVP were reduced in the brdx3 mutant back-ground. Finally, a brdx3 brm-3 quadruple mutant, in which SWI/SNF complexes were devoid of all constit-uent bromodomains, phenocopied a loss-of-function mutation in BRM. Taken together, our results demon-strate the relevance of BRDs as SWI/SNF subunits and suggest their cooperation with the bromodomain of BRM ATPase.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorIwanicka-Nowicka, Roksana
dc.contributor.authorKoblowska, Marta
dc.contributor.authorJarończyk, Kamila
dc.contributor.authorJerzmanowski, Andrzej
dc.contributor.authorBucholc, Maria
dc.contributor.authorPupel, Piotr
dc.contributor.authorSosnowska, Katarzyna
dc.contributor.authorMałecka, Ewelina
dc.contributor.authorSiwirykow, Nina
dc.contributor.authorZaborowski, Adam
dc.contributor.authorArchacki, Rafał
dc.contributor.authorStachula, Paulina
dc.date.accessioned2024-01-24T18:56:59Z
dc.date.available2024-01-24T18:56:59Z
dc.date.issued2021
dc.description.financeŚrodki finansowe przyznane na realizację projektu w zakresie badań naukowych lub prac rozwojowych
dc.description.number4
dc.description.volume2
dc.identifier.doi10.1016/J.XPLC.2021.100174
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102564
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S2590346221000511?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofPlant Communications
dc.relation.pages100174
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enArabidopsis
dc.subject.enchromatin remodeling
dc.subject.enSWI/SNF complex
dc.subject.engene regulation
dc.subject.enbromodomain-containing proteins
dc.subject.enBRD
dc.titleBromodomain-containing subunits BRD1, BRD2, and BRD13 are required for proper functioning of SWI/SNF complexes in Arabidopsis
dc.typeJournalArticle
dspace.entity.typePublication