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Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.

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cris.lastimport.scopus2024-02-12T19:46:09Z
dc.abstract.enNuclease and helicase activities play pivotal roles in various aspects of RNA processing and degradation. These two activities are often present in multi-subunit complexes from nucleic acid metabolism. In the mitochondrial exoribonuclease complex (mtEXO) both enzymatic activities are tightly coupled making it an excellent minimal system to study helicase–exoribonuclease coordination. mtEXO is composed of Dss1 3′-to-5′ exoribonuclease and Suv3 helicase. It is the master regulator of mitochondrial gene expression in yeast. Here, we present the structure of mtEXO and a description of its mechanism of action. The crystal structure of Dss1 reveals domains that are responsible for interactions with Suv3. Importantly, these interactions are compatible with the conformational changes of Suv3 domains during the helicase cycle. We demonstrate that mtEXO is an intimate complex which forms an RNA-binding channel spanning its entire structure, with Suv3 helicase feeding the 3′ end of the RNA toward the active site of Dss1.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorNowotny, Marcin
dc.contributor.authorKozak, Maciej
dc.contributor.authorNowak, Elżbieta
dc.contributor.authorDziembowski, Andrzej
dc.contributor.authorWarkocki, Zbigniew
dc.contributor.authorŁabędzka-Dmoch, Karolina
dc.contributor.authorTaube, Michał
dc.contributor.authorRażew, Michał
dc.contributor.authorCzarnocki-Cieciura, Mariusz
dc.contributor.authorPiątkowski, Jakub
dc.contributor.authorGolik, Paweł
dc.contributor.authorKawińska, Aleksandra
dc.contributor.authorKolondra, Adam
dc.date.accessioned2024-01-26T08:21:08Z
dc.date.available2024-01-26T08:21:08Z
dc.date.issued2018
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.description.volume9
dc.identifier.doi10.1038/S41467-017-02570-5
dc.identifier.issn2041-1723
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/120866
dc.identifier.weblinkhttps://www.nature.com/articles/s41467-017-02570-5
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofNature Communications
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleStructural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.
dc.typeJournalArticle
dspace.entity.typePublication