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Silver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis

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cris.lastimport.scopus2024-02-12T19:47:53Z
dc.abstract.enPurpose: Periodontal disease (PD), defined as oral inflammation caused by dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment options are limited. In this study, we designed, synthesized, and characterized silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or metronidazole (AgNPs-PEG-MET) to determine whether they can be used to treat PDs. Materials and Methods: AgNPs were synthesized and characterized by transmission electron microscopy, UV-vis spectrometry, thermogravimetric analyses, and dynamic light scattering. We determined the safety and the antimicrobial and anti-inflammatory properties of synthesized AgNPs in an in vitro model of periodontitis. Antimicrobial properties were determined by measuring the minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) on reference strains of bacteria and fungi. Human gingi-val fibroblast (HGF-1), murine macrophage (RAW264.7) and human foetal osteoblast (hFOB1.19) cells were used in the study. Lipopolysaccharide (LPS) was used to induce inflammation. Cytokine levels were measured using an enzyme-linked immunosorbent assay; metalloproteinase expression was measured using Western blotting. Results: The synthesized AgNPs were spherical and narrow-dispersed with an average diameter of 13.4 nm ± 3.0 nm in the case of AgNPs-CHL and 3.72 nm ± 0.72 nm in the case of AgNPs-PEG-MET. Both types of AgNPs were active against bacteria and fungi. AgNPs-CHL proved to be a more potent antimicrobial agent, although they were more cytotoxic than AgNPs-PEG-MET; however, both demonstrated beneficial properties in nontoxic concentrations. AgNPs-CHL and AgNPs-PEG-MET decreased the production of proinflammatory cytokines IL-1β, IL-6, IL-8 and TNFα. Both agents also decreased the levels of metalloproteinases MMP3 and MMP8, which may indicate that they will inhibit tissue degradation. Conclusion: AgNPs-CHL and AgNPs-PEG-MET may be possible therapeutic options for PD, as they have antibacterial and anti-inflammatory properties. However, to fully under-stand the potential of AgNPs, our in vitro findings must be evaluated in an in vivo model.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorInkielewicz-Stepniak, Iwona
dc.contributor.authorMegiel, Elżbieta
dc.contributor.authorKamysz, Wojciech
dc.contributor.authorBauer, Marta
dc.contributor.authorNarajczyk, Magdalena
dc.contributor.authorJaśkiewicz, Maciej
dc.contributor.authorBarcińska, Ewelina
dc.contributor.authorCieciórski, Piotr
dc.contributor.authorSteckiewicz, Karol P
dc.date.accessioned2024-01-26T07:36:54Z
dc.date.available2024-01-26T07:36:54Z
dc.date.copyright2022-02-02
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.versionFINAL_PUBLISHED
dc.description.volume17
dc.identifier.doi10.2147/IJN.S339046
dc.identifier.issn1176-9114
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/119817
dc.identifier.weblinkhttps://www.dovepress.com/getfile.php?fileID=78023
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational journal of nanomedicine
dc.relation.pages495-517
dc.rightsCC-BY-NC
dc.sciencecloudnosend
dc.subject.enDrug delivery platforms
dc.subject.enGingival fibroblast
dc.subject.enMacrophages
dc.subject.enOsteoblasts
dc.subject.enPeriodontal disease
dc.subject.enPeriodontitis
dc.titleSilver Nanoparticles as Chlorhexidine and Metronidazole Drug Delivery Platforms: Their Potential Use in Treating Periodontitis
dc.typeJournalArticle
dspace.entity.typePublication