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The RxLR motif of the host targeting effector AVR3a of Phytophthora infestans is cleaved before secretion.

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cris.lastimport.scopus2024-02-12T19:44:30Z
dc.abstract.enWhen plant-pathogenic oomycetes infect their hosts, they employ a large arsenal of effector proteins to establisha successful infection. Some effector proteins are secreted and are destined to be translocated and function inside host cells.The largest group of translocated proteins from oomycetes is the RxLR effectors, defined by their conserved N-terminal Arg-Xaa-Leu-Arg (RxLR) motif. However, the precise role of this motif in the host cell translocation process is unclear. Here,detailed biochemical studies of the RxLR effector AVR3a from the potato pathogenPhytophthora infestansare presented.Mass spectrometric analysis revealed that the RxLR sequence of native AVR3a is cleaved off prior to secretion by thepathogen and the N terminus of the mature effector was found likely to be acetylated. High-resolution NMR structure analysisof AVR3a indicates that the RxLR motif is well accessible to potential processing enzymes. Processing and modification ofAVR3a is to some extent similar to events occurring with the export element (PEXEL) found in malaria effector proteins fromPlasmodium falciparum. Thesefindings imply a role for the RxLR motif in the secretion of AVR3a by the pathogen, rather thana direct role in the host cell entry process itself.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBayer, Peter
dc.contributor.authorSecombes, Chris
dc.contributor.authorDavidson, Jan
dc.contributor.authorLinne, Uwe
dc.contributor.authorApostolakis, Kostis
dc.contributor.authorMatena, Anja
dc.contributor.authorTrusch, Franziska
dc.contributor.authorWest, Pieter van
dc.contributor.authorWawra, Stephan
dc.contributor.authorKoźmiński, Wiktor
dc.contributor.authorStanek, Jan
dc.contributor.authorŻukow, Igor
dc.date.accessioned2024-01-26T10:47:19Z
dc.date.available2024-01-26T10:47:19Z
dc.date.copyright2017-05-18
dc.date.issued2017
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number6
dc.description.versionFINAL_PUBLISHED
dc.description.volume29
dc.identifier.doi10.1105/TPC.16.00552
dc.identifier.issn1040-4651
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/123099
dc.identifier.weblinkhttps://academic.oup.com/plcell/article/29/6/1184/6099357
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofPlant Cell
dc.relation.pages1184-1195
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleThe RxLR motif of the host targeting effector AVR3a of Phytophthora infestans is cleaved before secretion.
dc.typeJournalArticle
dspace.entity.typePublication