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Identification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine

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cris.lastimport.scopus2024-02-12T19:44:38Z
dc.abstract.enBacteria of the genus Serratia inhabit a variety of ecological niches like water, soil, and the bodies of animals, and have a wide range of lifestyles. Currently, the complete genome sequences of 25 Serratia phages are available in the NCBI database. All of them were isolated from nutrient-rich environments like sewage, with the use of clinical Serratia strains as hosts. In this study, we identified a novel Serratia myovirus named vB_SspM_BZS1. Both the phage and its host Serratia sp. OS31 were isolated from the same oligotrophic environment, namely, an abandoned gold mine (Zloty Stok, Poland). The BZS1 phage was thoroughly characterized here in terms of its genomics, morphology, and infection kinetics. We also demonstrated that Serratia sp. OS31 was lysogenized by mitomycin-inducible siphovirus vB_SspS_OS31. Comparative analyses revealed that vB_SspM_BZS1 and vB_SspS_OS31 were remote from the known Serratia phages. Moreover, vB_SspM_BZS1 was only distantly related to other viruses. However, we discovered similar prophage sequences in genomes of various bacteria here. Additionally, a protein-based similarity network showed a high diversity of Serratia phages in general, as they were scattered across nineteen different clusters. In summary, this work broadened our knowledge on the diverse relationships of Serratia phages.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBujak, Katarzyna
dc.contributor.authorKaminski, Jerzy
dc.contributor.authorDecewicz, Przemysław
dc.contributor.authorRadlińska, Monika
dc.date.accessioned2024-01-25T03:35:01Z
dc.date.available2024-01-25T03:35:01Z
dc.date.issued2020
dc.description.financeŚrodki finansowe przyznane na realizację projektu w zakresie badań naukowych lub prac rozwojowych
dc.description.number18
dc.description.volume21
dc.identifier.doi10.3390/IJMS21186709
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108857
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/21/18/6709/pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages6709
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enbacteriophage
dc.subject.enprophage
dc.subject.entemperate virus
dc.subject.encomparative genomics
dc.subject.enSerratia
dc.subject.endam-like methyltransferase
dc.subject.ennon-specific DNA methyltransferase
dc.titleIdentification, Characterization, and Genomic Analysis of Novel Serratia Temperate Phages from a Gold Mine
dc.typeJournalArticle
dspace.entity.typePublication