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Apigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization

dc.abstract.enMineralization-competent cells like osteoblasts and chondrocytes release matrix vesicles (MVs) which accumulate Ca2+ and Pi, creating an optimal environment for apatite formation. The mineralization process requires the involvement of proteins, such as annexins (Anx) and tissue- nonspecific alkaline phosphatase (TNAP), as well as low molecular-weight compounds. Apigenin, a flavonoid compound, has been reported to affect bone metabolism, but there are doubts about its mechanism of action under physiological and pathological conditions. In this report, apigenin potency to modulate annexin A6 (AnxA6)- and TNAP-mediated osteoblast mineralization was explored using three cell lines: human fetal osteoblastic hFOB 1.19, human osteosarcoma Saos-2, and human coronary artery smooth muscle cells HCASMC. We compared the mineralization competence, the morphology and composition of minerals, and the protein distribution in control and apigenin-treated cells and vesicles. The mineralization ability was monitored by AR-S/CPC analysis, and TNAP activity was determined by ELISA assay. Apigenin affected the mineral structure and modulated TNAP activity depending on the concentration. We also observed increased mineralization in Saos-2 cells. Based on TEM-EDX, we found that apigenin influenced the mineral composition. This flavonoid also disturbed the intracellular distribution of AnxA6 and TNAP, especially blocking AnxA6 aggregation and TNAP attachment to the membrane, as examined by FM analysis of cells and TEM-gold analysis of vesicles. In summary, apigenin modulates the mineralization process by regulating AnxA6 and TNAP, as well as through various effects on normal and cancer bone tissues or atherosclerotic soft tissue
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBiesaga, Magdalena
dc.contributor.authorPIKUŁA, SŁAWOMIR
dc.contributor.authorKILISZEK, AGNIESZKA STRZELECKA -
dc.contributor.authorWeremiejczyk, Lilianna
dc.contributor.authorSUSKI, SZYMON
dc.contributor.authorMroczek, Joanna
dc.date.accessioned2024-01-24T16:44:03Z
dc.date.available2024-01-24T16:44:03Z
dc.date.copyright2022-10-29
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number21
dc.description.versionFINAL_PUBLISHED
dc.description.volume23
dc.identifier.doi10.3390/IJMS232113179
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100962
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/23/21/13179/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages13179
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleApigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization
dc.typeJournalArticle
dspace.entity.typePublication