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Porphyromonas gingivalis genes conferring fitness in a tobacco-rich environment

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cris.lastimport.scopus2024-02-12T19:45:20Z
dc.abstract.enSmokers are more likely than non-smokers to harbour Porphyromonas gingivalis, they are more susceptible to destructive periodontal disease and smokers may, ultimately, benefit from tobacco-specific preventive and treatment strategies. A Mariner transposon insertion library for P. gingivalis ATCC 33277 was exploited to define 256 genes as essential for P. gingivalis survival in a tobacco-rich environment. Genes whose products play roles in protein transport and catabolism, nicotinamide processing, protection against oxidative stress, drug resistance, and transcriptional regulation have all been identified as essential for CSE survival. Many of these tobacco-essential genes are also requisite for epithelial colonization and abscess formation, suggestive of a core stress-related P. gingivalis genome. Single-gene deletions in several of the TnSeq-implicated genes led to significantly reduced P. gingivalis fitness upon competition with the parent strain, under conditions of cigarette smoke extract-induced stress (1,000 ng/ml nicotine equivalents). This study identifies, for the first time, a subset of P. gingivalis genes required for surviving the plethora of insults present in cigarette smoke. Such conditionally essential genes may delineate bacterial persistence strategies and represent novel therapeutic foci for the prevention of P. gingivalis infection and related diseases in smokers and in general.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorŁasica, Anna
dc.contributor.authorLamont, Richard J.
dc.contributor.authorLiu, Chengcheng
dc.contributor.authorLamont, Gwyneth J.
dc.contributor.authorGogenini, Himabindu
dc.contributor.authorHutcherson, Justin A.
dc.contributor.authorScott, David A.
dc.contributor.authorMiller, Daniel P.
dc.contributor.authorPotempa, Jan
dc.contributor.authorNowakowska, Zuzanna
dc.contributor.authorYoder‐Himes, Deborah
dc.date.accessioned2024-01-25T17:16:19Z
dc.date.available2024-01-25T17:16:19Z
dc.date.issued2020
dc.date.openaccess1
dc.description.financePublikacja bezkosztowa
dc.description.number1
dc.description.volume35
dc.identifier.doi10.1111/OMI.12273
dc.identifier.issn2041-1006
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/116554
dc.identifier.weblinkhttps://ruj.uj.edu.pl/xmlui/handle/item/156940
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofMolecular Oral Microbiology
dc.relation.pages10-18
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enperiodontitis
dc.subject.enproteases
dc.subject.encigarette smoke
dc.subject.ennicotinamide
dc.subject.enoral bacteria
dc.subject.entransposon sequencing library
dc.titlePorphyromonas gingivalis genes conferring fitness in a tobacco-rich environment
dc.typeJournalArticle
dspace.entity.typePublication