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CD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis

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dc.abstract.enMycobacterial cell wall lipids bind the conserved CD1 family of antigen-presenting molecules and activate T cells via their T cell receptors (TCRs). Sulfoglycolipids (SGLs) are uniquely synthesized by Mycobacterium tuberculosis, but tools to study SGL-specific T cells in humans are lacking. We designed a novel hybrid synthesis of a naturally occurring SGL, generated CD1b tetramers loaded with natural or synthetic SGL analogs, and studied the molecular requirements for TCR binding and T cell activation. Two T cell lines derived using natural SGLs are activated by synthetic analogs independently of lipid chain length and hydroxylation, but differentially by saturation status. By contrast, two T cell lines derived using an unsaturated SGL synthetic analog were not activated by the natural antigen. Our data provide a bioequivalence hierarchy of synthetic SGL analogs and SGL-loaded CD1b tetramers. These reagents can now be applied to large-scale translational studies investigating the diagnostic potential of SGL-specific T cell responses or SGL-based vaccines.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPrandi, Jacques
dc.contributor.authorYedulla, Vijayendar R.
dc.contributor.authorMolęda, Zuzanna
dc.contributor.authorDiamanti, Eleonora
dc.contributor.authorKhan, Momin
dc.contributor.authorAggarwa, Varinder K.
dc.contributor.authorReijneveld, Josephine F.
dc.contributor.authorReinink, Peter
dc.contributor.authorLenz, Stefanie
dc.contributor.authorEmerson, Ryan O.
dc.contributor.authorScriba, Thomas J.
dc.contributor.authorSouter, Michael N.T.
dc.contributor.authorGodfrey, Dale I.
dc.contributor.authorPellicci, Daniel G.
dc.contributor.authorMinnaard, Adriaan J.
dc.contributor.authorSeshadri, Chetan
dc.contributor.authorRhijn, Ildiko Van
dc.contributor.authorJames, Charlotte A.
dc.contributor.authorYu, Krystle K.Q.
dc.contributor.authorGilleron, Martine
dc.contributor.authorMoody, D. Branch
dc.date.accessioned2024-01-24T19:04:46Z
dc.date.available2024-01-24T19:04:46Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number4
dc.description.volume25
dc.identifier.doi10.1016/J.CHEMBIOL.2018.01.006
dc.identifier.issn2451-9448
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102777
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofCell Chemical Biology
dc.relation.pages392-402
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enhuman
dc.subject.enT cells
dc.subject.enmycobacteria
dc.subject.entuberculosis
dc.subject.enantigen-presentation
dc.subject.enCD1
dc.subject.enT cell receptor
dc.subject.enlipid antigen
dc.titleCD1b Tetramers Identify T Cells that Recognize Natural and Synthetic Diacylated Sulfoglycolipids from Mycobacterium tuberculosis
dc.typeJournalArticle
dspace.entity.typePublication