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HPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress

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dc.abstract.enTo cope with environmental harmful conditions, plant cells developed adaptive strategy that involves production of a wide variety of complex secondary metabolites. The spectrum and quantity of biosynthesized compounds in specific plant species is determined by its genotype, tissue, developmental and physiological stage and environmental factors. This phenomenon was used to exploit the potential of anatomical and hairy root cultures of Rindera graeca to produce bioactive compounds. Cultivated in vitro roots were subjected to abiotic stresses i.e., drought or coldness. Next the extract profiling was performed using HPLC-PDA-ESI-HRMS method, as well quantitative determination of caffeic, rosmarinic and lithospermic B acids, that were present in all root extracts. Phenolic acids, flavonoids and iridoids represent the major groups of compounds detected in chemical profiles growing under various conditions roots. The highest number of phytochemicals was determined in roots subjected to coldness. Lithospermic B acid proved to be the most abundant compound in all investigated extracts. Among applied abiotic stress factors it was demonstrated that coldness affected to the most secondary metabolites production. The results of current study suggest that root cultures of R. graeca could serve as a new and abundant source of lithospermic B acid.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorSykłowska-Baranek, Katarzyna
dc.contributor.authorPietrosiuk, Agnieszka
dc.contributor.authorMisicka-Kęsik, Aleksandra
dc.contributor.authorWileńska, Beata
dc.contributor.authorNaliwajski, Marcin Robert
dc.date.accessioned2024-01-25T03:30:39Z
dc.date.available2024-01-25T03:30:39Z
dc.date.copyright2022-03-08
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number6
dc.description.versionFINAL_PUBLISHED
dc.description.volume11
dc.identifier.doi10.3390/CELLS11060931
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/108716
dc.identifier.weblinkhttps://www.mdpi.com/2073-4409/11/6/931/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofCells
dc.relation.pages931
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enAbiotic stresses
dc.subject.enBoraginaceae rosmarinic acid
dc.subject.enChemical profile
dc.subject.enIn vitro cultures
dc.subject.enLithospermic B acid
dc.subject.enTotal phenolic and total flavonoid content
dc.titleHPLC-PDA-ESI-HRMS-Based Profiling of Secondary Metabolites of Rindera graeca Anatomical and Hairy Roots Treated with Drought and Cold Stress
dc.typeJournalArticle
dspace.entity.typePublication