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Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

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cris.lastimport.scopus2024-02-12T19:58:38Z
dc.abstract.enSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of spike protein to the host cell surface-expressing angiotensin-converting enzyme 2 (ACE2) or by endocytosis mediated by extracellular matrix metalloproteinase inducer (CD147). We present extended statistical studies of the multisine dynamic electrochemical impedance spectroscopy (DEIS) revealing interactions between Spike RBD and cellular receptors ACE2 and CD147, and a reference anti-RBD antibody (IgG2B) based on a functionalised boron-doped diamond (BDD) electrode. The DEIS was supported by a multivariate data analysis of a SARS-CoV-2 Spike RBD assay and cross-correlated with the atomic-level information revealed by molecular dynamics simulations. This approach allowed us to study and detect subtle changes in the electrical properties responsible for the susceptibility of cellular receptors to SARS-CoV-2, revealing their interactions. Changes in electrical homogeneity in the function of the RBD concentration led to the conclusion that the ACE2 receptor delivers the most homogeneous surface, delivered by the high electrostatic potential of the relevant docking regions. For higher RBD concentrations, the differences in electrical homogeneity between electrodes with different receptors vanish. Collectively, this study reveals interdependent virus entry pathways involving separately ACE2, CD147, and spike protein, as assessed using a biosensing platform for the rapid screening of cellular interactions (i.e. testing various mutations of SARS-CoV-2 or screening of therapeutic drugs).
dc.affiliationUniwersytet Warszawski
dc.contributor.authorTrzaskowski, Bartosz
dc.contributor.authorBogdanowicz, Robert
dc.contributor.authorRyl, Jacek
dc.contributor.authorSwain, Greg N.
dc.contributor.authorŚlepski, Paweł
dc.contributor.authorFicek, Mateusz
dc.contributor.authorKowalski, Marcin
dc.contributor.authorJanik, Monika
dc.contributor.authorPierpaoli, Mattia
dc.contributor.authorBrodowski, Mateusz
dc.date.accessioned2024-01-24T22:48:24Z
dc.date.available2024-01-24T22:48:24Z
dc.date.issued2022
dc.description.financePublikacja bezkosztowa
dc.description.volume370
dc.identifier.doi10.1016/J.SNB.2022.132427
dc.identifier.issn0925-4005
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/106223
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofSensors and Actuators, B: Chemical
dc.relation.pages132427
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enBIOSENSORS
dc.subject.enDEIS
dc.subject.enELECTROCHEMICAL IMMUNOSENSOR
dc.subject.enSARS-COV-2
dc.titleEnhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response
dc.typeJournalArticle
dspace.entity.typePublication