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Exosomes derived from lung cancer cells: Isolation, characterization, and stability studies

Uproszczony widok
dc.abstract.enRecent advances in nanomedicine have paved the way for developing targeted drug delivery systems. Nanoscale exosomes are present in almost every body fluid and represent a novel mechanism of intercellular communication. Because of their membrane origin, they easily fuse with cells, acting as a natural delivery system and maintaining the bioactivity and immunotolerance of cells. To develop a reconstitutable exosome-based drug candidate for clinical applications, quality assurance by preserving its physical and biological properties during storage is necessary. Therefore, this study aimed to determine the best storage conditions for exosomes derived from lung cancer cells (A549). This study established that the phosphate-buffered saline buffer enriched with 25 mM trehalose is an optimal cryoprotectant for A549-derived exosomes stored at −80°C. Under these conditions, the concentration, size distribution, zeta potential, and total cargo protein levels of the preserved exosomes remained constant
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGrudziński, ireneusz
dc.contributor.authorDonten, Mikołaj
dc.contributor.authorSobczak, Kamil
dc.contributor.authorMOSIENIAK, GRAŻYNA
dc.contributor.authorTargońska, Alicja
dc.contributor.authorGłuchowska, Agata
dc.contributor.authorKowalczyk, Agata
dc.contributor.authorNowicka, Anna
dc.contributor.authorRuzycka-Ayoush, Monika
dc.date.accessioned2024-01-25T00:07:06Z
dc.date.available2024-01-25T00:07:06Z
dc.date.copyright2022-12-24
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.versionFINAL_PUBLISHED
dc.description.volume181
dc.identifier.doi10.1016/J.EJPS.2022.106369
dc.identifier.issn0928-0987
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/106720
dc.identifier.weblinkhttps://api.elsevier.com/content/article/PII:S0928098722002548?httpAccept=text/xml
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofEuropean Journal of Pharmaceutical Sciences
dc.relation.pages106369
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.subject.enExosome
dc.subject.enLung cancer
dc.subject.enPreservatives
dc.subject.enStability
dc.subject.enStorage conditions
dc.titleExosomes derived from lung cancer cells: Isolation, characterization, and stability studies
dc.typeJournalArticle
dspace.entity.typePublication