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Molecular Imaging of Human Skeletal Myoblasts (huSKM) in Mouse Post-Infarction Myocardium

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cris.lastimport.scopus2024-02-12T20:34:18Z
dc.abstract.en<jats:p>Current treatment protocols for myocardial infarction improve the outcome of disease to some extent but do not provide the clue for full regeneration of the heart tissues. An increasing body of evidence has shown that transplantation of cells may lead to some organ recovery. However, the optimal stem cell population has not been yet identified. We would like to propose a novel pro-regenerative treatment for post-infarction heart based on the combination of human skeletal myoblasts (huSkM) and mesenchymal stem cells (MSCs). huSkM native or overexpressing gene coding for Cx43 (huSKMCx43) alone or combined with MSCs were delivered in four cellular therapeutic variants into the healthy and post-infarction heart of mice while using molecular reporter probes. Single-Photon Emission Computed Tomography/Computed Tomography (SPECT/CT) performed right after cell delivery and 24 h later revealed a trend towards an increase in the isotopic uptake in the post-infarction group of animals treated by a combination of huSkMCx43 with MSC. Bioluminescent imaging (BLI) showed the highest increase in firefly luciferase (fluc) signal intensity in post-infarction heart treated with combination of huSkM and MSCs vs. huSkM alone (p &lt; 0.0001). In healthy myocardium, however, nanoluciferase signal (nanoluc) intensity varied markedly between animals treated with stem cell populations either alone or in combinations with the tendency to be simply decreased. Therefore, our observations seem to show that MSCs supported viability, engraftment, and even proliferation of huSkM in the post-infarction heart.</jats:p>
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKolasiński, Jerzy
dc.contributor.authorBorkowski, Szymon
dc.contributor.authorHamankiewicz, Paulina
dc.contributor.authorFiedorowicz, Katarzyna
dc.contributor.authorWargocka-Matuszewska, Weronika
dc.contributor.authorAmbrożkiewicz, Karolina
dc.contributor.authorRugowska, Anna
dc.contributor.authorCheda, Łukasz
dc.contributor.authorFiedorowicz, Michał
dc.contributor.authorZimna, Agnieszka
dc.contributor.authorDrabik, Monika
dc.contributor.authorŚwiątkiewicz, Maciej
dc.contributor.authorBogorodzki, Piotr
dc.contributor.authorGrieb, Paweł
dc.contributor.authorKolanowski, Tomasz
dc.contributor.authorRozwadowska, Natalia
dc.contributor.authorKozłowska, Urszula
dc.contributor.authorKlimczak, Aleksandra
dc.contributor.authorRogulski, Zbigniew
dc.contributor.authorKurpisz, Maciej
dc.date.accessioned2024-01-25T12:53:16Z
dc.date.available2024-01-25T12:53:16Z
dc.date.issued2021
dc.description.financePublikacja bezkosztowa
dc.description.number19
dc.description.volume22
dc.identifier.doi10.3390/IJMS221910885
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/112861
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages1-20
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enhuman skeletal myoblasts
dc.subject.enmesenchymal stem cells
dc.subject.enSingle-Photon Emission Computed Tomography/Computed Tomography
dc.subject.enMagnetic Resonance Imaging
dc.subject.enBioluminescent Imaging
dc.subject.enpromoter reporter gene
dc.subject.entechnetium
dc.titleMolecular Imaging of Human Skeletal Myoblasts (huSKM) in Mouse Post-Infarction Myocardium
dc.typeJournalArticle
dspace.entity.typePublication