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LDL dinitrosyl iron complex acts as an iron donor in mouse macrophages

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dc.abstract.en[Fe(NO)2] – modified nanoparticles of low-density protein (DNICLDL) can serve as conveyors of iron in the form of stable complexes with ApoB100 protein. As reported recently, in human hepatoma cells DNICLDL significantly increased the total iron content, while showing low toxicity. In the present work, we focused on the effects of internalization of DNIC-modified lipoproteins in macrophages, with special regards to cytotoxicity. DNICLDL was administered to a model macrophage cell line, RAW 264.7. Administration of DNICLDL considerably increased total iron content. High increase of iron was accompanied by moderate toxicity. As shown by in vitro plasmid nicking assay, chelation of iron in the form of DNIC strongly reduced the iron-related reactive oxygen species (ROS) -induced DNA damage. In addition, DNICLDL, plausibly due to its NO-donating activity, did not induce inducible nitric oxide synthase (iNOS) expression, as opposed to other forms of low-density protein (LDL).
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDudek, Jakub
dc.contributor.authorStępkowski, Tomasz
dc.contributor.authorKruszewski, Marcin
dc.contributor.authorSikorska, Katarzyna
dc.contributor.authorSadło, Jarosław
dc.contributor.authorLewandowska-Siwkiewicz, Hanna
dc.contributor.authorMęczyńska-Wielgosz, Sylwia
dc.date.accessioned2024-01-25T05:08:00Z
dc.date.available2024-01-25T05:08:00Z
dc.date.issued2018
dc.description.financeNie dotyczy
dc.description.number11
dc.description.volume188
dc.identifier.doi10.1016/J.JINORGBIO.2018.08.004
dc.identifier.issn0162-0134
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/111011
dc.identifier.weblinkhttps://www.sciencedirect.com/science/article/pii/S0162013418301971
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofJournal of Inorganic Biochemistry
dc.relation.pages29-37
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleLDL dinitrosyl iron complex acts as an iron donor in mouse macrophages
dc.typeJournalArticle
dspace.entity.typePublication