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Deletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII

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dc.abstract.enWe have successfully produced single-cell colonies of C. merolae mutants, lacking the PsbQ' subunit in its PSII complex by application of DTA-aided mutant selection. We have investigated the physiological changes in PSII function and structure and proposed a tentative explanation of the function of PsbQ' subunit in the PSII complex. We have improved the selectivity of the Cyanidioschyzon merolae nuclear transformation method by the introduction of diphtheria toxin genes into the transformation vector as an auxiliary selectable marker. The revised method allowed us to obtained single-cell colonies of C. merolae, lacking the gene of the PsbQ' extrinsic protein. The efficiency of gene replacement was extraordinarily high, allowing for a complete deletion of the gene of interest, without undesirable illegitimate integration events. We have confirmed the absence of PsbQ' protein at genetic and protein level. We have characterized the physiology of mutant cells and isolated PSII protein complex and concluded that PsbQ' is involved in nuclear regulation of PSII activity, by influencing several parameters of PSII function. Among these: oxygen evolving activity, partial dissociation of PsbV, regulation of dimerization, downsizing of phycobilisomes rods and regulation of zeaxanthin abundance. The adaptation of cellular physiology appeared to favorite upregulation of PSII and concurrent downregulation of PSI, resulting in an imbalance of energy distribution, decrease of photosynthesis and inhibition of cell proliferation.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGolke, Anna
dc.contributor.authorKrupnik, Tomasz
dc.contributor.authorRomanowska, Elżbieta
dc.contributor.authorWasilewska-Dębowska, Wioleta
dc.contributor.authorZienkiewicz, Maksymilian
dc.contributor.authorDrożak, Anna
dc.date.accessioned2024-01-24T21:19:44Z
dc.date.available2024-01-24T21:19:44Z
dc.date.copyright2017-12-01
dc.date.issued2018
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.identifier.doi10.1007/S11103-017-0685-6
dc.identifier.issn0167-4412
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/104495
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofPlant Molecular Biology
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enChloramphenicol acetyltransferase
dc.subject.enCyanidioschyzon merolae
dc.subject.enDTA toxin
dc.subject.enPEG
dc.subject.enPSII extrinsic protein deletion
dc.subject.enPSII mutants
dc.subject.enPsbQ’
dc.subject.enStable red algae nuclear transformation
dc.titleDeletion of psbQ’ gene in Cyanidioschyzon merolae reveals the function of extrinsic PsbQ’ in PSII
dc.typeJournalArticle
dspace.entity.typePublication