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Biofunctionalisation of p-doped silicon with cytochrome c_{553} minimises charge recombination and enhances photovoltaic performance of the all-solid-state photosystem I-based biophotoelectrode

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cris.lastimport.scopus2024-02-12T20:52:49Z
dc.abstract.enSurface-directed passivation of p-doped silicon (Si) substrate was achieved by its biofunctionalisation with hexahistidine (His6)-tagged cytochrome c553 (cyt c553), a soluble electroactive photosynthetic protein responsible for electron donation to photooxidised photosystem I (PSI). Five distinct variants of cyt c553 were genetically engineered by introducing the specific linker peptides of 0-19 amino acids (AA) in length between the cyt c553 holoprotein and a C-terminal His6-tag, the latter being the affinity 'anchor' used for the specific immobilisation of this protein on the semiconductor surface. Calculation of 2D Gibbs free energy maps for the five cyt c553 variants showed a significantly higher number of thermodynamically feasible conformations of immobilised cyt c variants containing longer linker peptides. Here we show that the distinct cyt c553-based Si bioelectrodes display some characteristics of the p-n-type diodes, albeit varying in the level of dark saturation current J0 considered as the charge recombination parameter. These combined bioinformatic and electrochemical analyses indicate that the cyt c553 variants with longer linker peptides, up to 19AA in length, allow for more structural flexibility of immobilised cyt c553 in terms of both, orientation and distance of the haem group with respect to the Si surface, and promote the efficient biopassivation of the semiconductor substrate. Incorporation of the specifically immobilised 19AA cyt c553 variant into the all-solid-state biophotoelectrodes containing light harvesting PSI module enhanced biophotovoltaic performance of the PSI biophotoelectrode compared to the analogous device devoid of cyt c553
dc.affiliationUniwersytet Warszawski
dc.contributor.authorOlmos, Julian Janna
dc.contributor.authorOlmos, Julian David Janna
dc.contributor.authorBecquet, Philippe
dc.contributor.authorGront, Dominik
dc.contributor.authorSar, Jarosław
dc.contributor.authorDąbrowski, Andrzej
dc.contributor.authorGawlik, Grzegorz
dc.contributor.authorTeodorczyk, Marian
dc.contributor.authorKargul, Joanna
dc.contributor.authorPAWLAK, DOROTA
dc.date.accessioned2024-01-24T18:39:03Z
dc.date.available2024-01-24T18:39:03Z
dc.date.copyright2017-10-11
dc.date.issued2017
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number75
dc.description.versionFINAL_PUBLISHED
dc.description.volume7
dc.identifier.doi10.1039/C7RA10895H
dc.identifier.issn2046-2069
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102323
dc.languageeng
dc.relation.ispartofRSC Advances
dc.relation.pages47854-47866
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleBiofunctionalisation of p-doped silicon with cytochrome c_{553} minimises charge recombination and enhances photovoltaic performance of the all-solid-state photosystem I-based biophotoelectrode
dc.typeJournalArticle
dspace.entity.typePublication