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PEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae

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dc.abstract.enThe ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of Cyanidioschizon merolae in both nuclear and chloroplast genome by means of homologous recombination between the genome and a transformation vector. The use of chloramphenicol resistance gene as the selectable marker allows for plate-based efficient selection of mutant colonies. Overall, the method allows the generation of mutant strains within 6 months.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKania, Kinga
dc.contributor.authorZienkiewicz, Maksymilian
dc.contributor.authorKrupnik, Tomasz
dc.contributor.authorDrożak, Anna
dc.date.accessioned2024-01-25T16:22:41Z
dc.date.available2024-01-25T16:22:41Z
dc.date.copyright2019-09-17
dc.date.issued2019
dc.date.openaccess1
dc.description.accesstimeAFTER_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number17
dc.description.versionFINAL_PUBLISHED
dc.description.volume9
dc.identifier.doi10.21769/BIOPROTOC.3355
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115652
dc.identifier.weblinkhttp://dx.doi.org/10.21769/bioprotoc.3355
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofBio-protocol
dc.relation.pages607-611
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.entransformation
dc.titlePEG-mediated, Stable, Nuclear and Chloroplast Transformation of Cyanidioschizon merolae
dc.typeJournalArticle
dspace.entity.typePublication