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Characterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent

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dc.abstract.enBordetella bronchiseptica, an emerging zoonotic pathogen, infects a broad range of mammalian hosts. B. bronchiseptica-associated atrophic rhinitis incurs substantial losses to the pig breeding industry. The true burden of human disease caused by B. bronchiseptica is unknown, but it has been postulated that some hypervirulent B. bronchiseptica isolates may be responsible for undiagnosed respiratory infections in humans. B. bronchiseptica was shown to acquire antibiotic resistance genes from other bacterial genera, especially Escherichia coli. Here, we present a new B. bronchiseptica lytic bacteriophage-vB_BbrP_BB8-of the Podoviridae family, which offers a safe alternative to antibiotic treatment of B. bronchiseptica infections. We explored the phage at the level of genome, physiology, morphology, and infection kinetics. Its therapeutic potential was investigated in biofilms and in an in vivo Galleria mellonella model, both of which mimic the natural environment of infection. The BB8 is a unique phage with a genome structure resembling that of T7-like phages. Its latent period is 75 ± 5 min and its burst size is 88 ± 10 phages. The BB8 infection causes complete lysis of B. bronchiseptica cultures irrespective of the MOI used. The phage efficiently removes bacterial biofilm and prevents the lethality induced by B. bronchiseptica in G. mellonella honeycomb moth larvae.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorOrszagh-Szturo, H.
dc.contributor.authorDołęgowska, Barbara
dc.contributor.authorPankowski, Jarosław
dc.contributor.authorGolec, Piotr
dc.contributor.authorKarczewska-Golec, Joanna
dc.contributor.authorSzymczak, M.
dc.contributor.authorBącal, Paweł
dc.contributor.authorDecewicz, Przemysław
dc.contributor.authorGrygorcewicz, Bartłomiej
dc.date.accessioned2024-01-24T19:06:49Z
dc.date.available2024-01-24T19:06:49Z
dc.date.issued2020
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.number4
dc.description.volume21
dc.identifier.doi10.3390/IJMS21041403
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/102897
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages1403
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enGalleria mellonella
dc.subject.enanimal model
dc.subject.enantibiotic resistance
dc.subject.enatrophic rhinitis
dc.subject.enbiofilm
dc.subject.enemerging diseases
dc.subject.enphage stability
dc.subject.enphage therapy
dc.subject.enveterinary microbiology
dc.subject.enzoonosis
dc.titleCharacterization of a Unique Bordetella bronchiseptica vB_BbrP_BB8 Bacteriophage and Its Application as an Antibacterial Agent
dc.typeJournalArticle
dspace.entity.typePublication