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Dostęp zamkniętyCryoprotection and banking of living cells in a 3D multiple emulsion-based carrier
| dc.abstract.en | The ability to preserve stem cells/cells with minimal damage for short and long periods of time is essential for advancements in biomedical therapies and biotechnology. New methods of cell bank- ing are continuously needed to provide effective damage prevention to cells. This paper puts for- ward a solution to the problem of the low viability of cells during cryopreservation in a traditional suspension and storage by developing innovative multiple emulsion-based carriers for the encap- sulation and cryopreservation of cells. During freezing-thawing processes, irreversible damage to cells occurs as a result of the formation of ice crystals, cell dehydration, and the toxicity of cryo- protectant. The proposed method was effective due to the “flexible” protective structure of multi- ple emulsions, which was proven by a high cell survival rate, above 90%. Results make new con- tributions in the fields of cell engineering and biotechnology and contribute to the development of methods for banking biological material. |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Kosicki, Konrad |
| dc.contributor.author | Markowska-Radomska, Agnieszka |
| dc.contributor.author | Cui, Zhanfeng |
| dc.contributor.author | Dłuska, Ewa |
| dc.contributor.author | Metera, Agata |
| dc.date.accessioned | 2024-01-24T20:55:39Z |
| dc.date.available | 2024-01-24T20:55:39Z |
| dc.date.issued | 2017 |
| dc.description.finance | Nie dotyczy |
| dc.description.volume | 12 |
| dc.identifier.doi | 10.1002/BIOT.201600692 |
| dc.identifier.issn | 1860-6768 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/103983 |
| dc.identifier.weblink | http://onlinelibrary.wiley.com/doi/10.1002/biot.201600692/epdf |
| dc.language | eng |
| dc.pbn.affiliation | biological sciences |
| dc.relation.ispartof | Biotechnology journal |
| dc.relation.pages | 1-7 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | cells |
| dc.subject.en | cell viability |
| dc.subject.en | cryopreservation |
| dc.subject.en | encapsulation |
| dc.subject.en | multiple emulsion |
| dc.title | Cryoprotection and banking of living cells in a 3D multiple emulsion-based carrier |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |