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Multiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([18F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers

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dc.abstract.enCardiovascular diseases (CVD), with myocardial infarction (MI) being one of the crucial components, wreak havoc in developed countries. Advanced imaging technologies are required to obtain quick and widely available diagnostic data. This paper describes a multimodal approach to in vivo perfusion imaging using the novel SYN1 tracer based on the fluorine‐18 isotope. The NOD‐ SCID mice were injected intravenously with SYN1 or [18F] fluorodeoxyglucose ([18F]‐FDG) radiotracers after induction of the MI. In all studies, the positron emission tomography–computed tomography (PET/CT) technique was used. To obtain hemodynamic data, mice were subjected to magnetic resonance imaging (MRI). Finally, the biodistribution of the SYN1 compound was performed using Wistar rat model. SYN1 showed normal accumulation in mouse and rat hearts, and MI hearts correctly indicated impaired cardiac segments when compared to [18 F]‐FDG uptake. In vivo PET/CT and MRI studies showed statistical convergence in terms of the size of the necrotic zone and cardiac function. This was further supported with RNAseq molecular analyses to correlate the candidate function genes’ expression, with Serpinb1c, Tnc and Nupr1, with Trem2 and Aldolase B functional correlations showing statistical significance in both SYN1 and [18 F]‐FDG. Our manuscript presents a new fluorine‐18‐based perfusion radiotracer for PET/CT imaging that may have importance in clinical applications. Future research should focus on confirmation of the data elucidated here to prepare SYN1 for first‐in‐human trials
dc.affiliationUniwersytet Warszawski
dc.contributor.authorRugowska, Anna
dc.contributor.authorZyprych-Walczak, Joanna
dc.contributor.authorKolanowski, Tomasz Jan
dc.contributor.authorCheda, Lukasz
dc.contributor.authorZimna, Agnieszka
dc.contributor.authorDrabik, Monika
dc.contributor.authorWargocka-Matuszewska, Weronika
dc.contributor.authorSteczek, Łukasz
dc.contributor.authorRogulski, Zbigniew
dc.contributor.authorKrajewski, Seweryn
dc.contributor.authorFiedorowicz, Michał
dc.contributor.authorKurpisz, Maciej
dc.contributor.authorRozwadowska, Natalia
dc.contributor.authorKozanecki, Cezary
dc.date.accessioned2024-01-25T13:12:43Z
dc.date.available2024-01-25T13:12:43Z
dc.date.copyright2021-11-22
dc.date.issued2021
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number22
dc.description.versionFINAL_PUBLISHED
dc.description.volume22
dc.identifier.doi10.3390/IJMS222212591
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113106
dc.identifier.weblinkhttps://www.mdpi.com/1422-0067/22/22/12591/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages12591
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enFDG
dc.subject.enFluorine radiotracers
dc.subject.enMolecular profiling after myocardial infarction
dc.subject.enPositron emission tomography–computed tomography (PET/CT)
dc.subject.enRNAseq of the heart tissue
dc.subject.enSYN1
dc.subject.en[18F]‐FDG
dc.titleMultiparametric Evaluation of Post-MI Small Animal Models Using Metabolic ([18F]FDG) and Perfusion-Based (SYN1) Heart Viability Tracers
dc.typeJournalArticle
dspace.entity.typePublication