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1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning

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dc.abstract.enSince the first pioneering studies on small deuterated peptides dating more than 20 years ago, 1H detection has evolved into the most efficient approach for investigation of biomolecular structure, dynamics, and interactions by solid-state NMR. The development of faster and faster magic-angle spinning (MAS) rates (up to 150 kHz today) at ultrahigh magnetic fields has triggered a real revolution in the field. This new spinning regime reduces the 1H–1H dipolar couplings, so that a direct detection of 1H signals, for long impossible without proton dilution, has become possible at high resolution. The switch from the traditional MAS NMR approaches with 13C and 15N detection to 1H boosts the signal by more than an order of magnitude, accelerating the site-specific analysis and opening the way to more complex immobilized biological systems of higher molecular weight and available in limited amounts. This paper reviews the concepts underlying this recent leap forward in sensitivity and resolution, presents a detailed description of the experimental aspects of acquisition of multidimensional correlation spectra with fast MAS, and summarizes the most successful strategies for the assignment of the resonances and for the elucidation of protein structure and conformational dynamics. It finally outlines the many examples where 1H-detected MAS NMR has contributed to the detailed characterization of a variety of crystalline and noncrystalline biomolecular targets involved in biological processes ranging from catalysis through drug binding, viral infectivity, amyloid fibril formation, to transport across lipid membranes.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPintacuda, Guido
dc.contributor.authorStanek, Jan
dc.contributor.authorJaudzems, Kristaps
dc.contributor.authorAndreas, Loren B.
dc.contributor.authorPell, Andrew J.
dc.contributor.authorLalli, Daniela
dc.contributor.authorPaluch, Piotr
dc.contributor.authorBonaccorsi, Marta
dc.contributor.authorSchubeis, Tobias
dc.contributor.authorMarchand, Tanguy Le
dc.date.accessioned2024-01-24T15:35:26Z
dc.date.available2024-01-24T15:35:26Z
dc.date.copyright2022-05-10
dc.date.issued2022
dc.description.accesstimeBEFORE_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number10
dc.description.versionFINAL_PUBLISHED
dc.description.volume122
dc.identifier.doi10.1021/ACS.CHEMREV.1C00918
dc.identifier.issn0009-2665
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/100027
dc.identifier.weblinkhttps://pubs.acs.org/doi/pdf/10.1021/acs.chemrev.1c00918
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofChemical Reviews
dc.relation.pages9943-10018
dc.rightsCC-BY-NC-ND
dc.sciencecloudnosend
dc.title1H-Detected Biomolecular NMR under Fast Magic-Angle Spinning
dc.typeJournalArticle
dspace.entity.typePublication