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Enzymatic digestion of luminescent albumin-stabilized gold nanoclusters under anaerobic conditions: clues to the quenching mechanism

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dc.abstract.enMany of the potential applications of albumin-stabilized gold nanoclusters (AuNCs) arise from the sensitivity of their luminescence to the presence of various ions and albumin-degrading proteases. However, the underlying photophysics and the mechanisms responsible for protease-induced quenching of AuNC luminescence are not fully understood. Here, we study proteinase K-induced digestion of a bovine serum albumin (BSA)-AuNC conjugate under aerobic and anaerobic conditions. To this end, we adapt a Co(II)-catalyzed sulfite-based protocol enabling effective in situ deoxidization without deactivation of the enzyme. In the absence of proteinase K, the anaerobic conditions facilitate the luminescence of BSA-AuNC reflected by a moderate increase in the red luminescence intensity. However, in the presence of proteinase K, we have observed a steep decrease of the emission intensity, irrespective of whether the digestion was carried out under aerobic or anaerobic conditions. In both cases, a decrease of fluorescence occurred "in phase" with shifting of the emission maximum to longer wavelengths. These results contradict the previous hypothesis that protease-induced quenching of BSA-AuNC luminescence is a consequence of enhanced diffusion of oxygen to the bare AuNC. Protease-mediated removal of certain amino acids (e.g. tyrosine) from the vicinity of the AuNCs followed by agglomeration of the AuNCs into non-luminescent assemblies underlies the quenching process. Our findings are discussed in the context of the mechanisms of formation and the photophysics of BSA-AuNC conjugates.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorDzwolak, Wojciech
dc.contributor.authorNIEZNAŃSKA, HANNA
dc.contributor.authorWacławska, Matylda
dc.date.accessioned2024-01-24T22:49:27Z
dc.date.available2024-01-24T22:49:27Z
dc.date.issued2022
dc.description.financePublikacja bezkosztowa
dc.description.number10
dc.description.volume10
dc.identifier.doi10.1039/D1TC05891F
dc.identifier.issn2050-7534
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/106309
dc.identifier.weblinkhttp://pubs.rsc.org/en/content/articlepdf/2022/TC/D1TC05891F
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofJournal of Materials Chemistry C
dc.relation.pages3775-3783
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleEnzymatic digestion of luminescent albumin-stabilized gold nanoclusters under anaerobic conditions: clues to the quenching mechanism
dc.typeJournalArticle
dspace.entity.typePublication