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Renal carcinoma CD105-/CD44-cells display stem-like properties in vitro and form aggressive tumors in vivo.

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dc.abstract.enClear cell renal cell carcinoma (ccRCC) is the most common kidney cancer. Prognosis for ccRCC is generally poor since it is largely resistant to chemo- and radiotherapy. Many studies suggested that cancer stem cells/tumor initiating cells (CSCs/TICs) are responsible for development of tumor, disease progression, aggressiveness, metastasis and drug resistance. However, tumorigenic potential of CSCs/TICs isolated from established RCC cell lines – basic ccRCC research model – has never been investigated in vivo. CD105+, CD105−, CD44+ and CD44− as well as CD44−/CD105− CD44+/CD105+ and CD44−/CD105+ cells were isolated from Caki-1 RCC cell line, confirming coexistence of multiple subpopulations of stem-related phenotype in stable cell line. Sorted cells were injected subcutaneously into NOD SCID mice and tumor growth was monitored with MRI and PET/CT. Tumor growth was observed after implantation of CD105+, CD44+, CD44−, CD44−/CD105+ and CD44−/CD105− but not CD105− or CD44+/CD105+. Implantation of CD44−/CD105− cells induced tumors that were characterized by longer T1 and distinct metabolic pattern than other tumors. All the tumors were characterized by low uptake of [18F]FDG. CD105+ and CD44− tumors expresses Nanog and Oct-4, while CD44− tumors additionally expressed endothelial cell marker - CD31.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorFiedorowicz, Michał
dc.contributor.authorWargocka-Matuszewska, Weronika Anna
dc.contributor.authorCheda, Łukasz
dc.contributor.authorKhan, M.I.
dc.contributor.authorSobiborowicz, Małgorzata
dc.contributor.authorSzczylik, Cezary
dc.contributor.authorWełniak-Kamińska, Marlena
dc.contributor.authorWieteska, Michał
dc.contributor.authorRogulski, Zbigniew
dc.contributor.authorKilian, Krzysztof
dc.contributor.authorStrzemecki, Damian
dc.contributor.authorCzarnecka, Anna
dc.contributor.authorOrzeł, Jarosław
dc.date.accessioned2024-01-25T19:11:36Z
dc.date.available2024-01-25T19:11:36Z
dc.date.copyright2020-03-25
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number(1)
dc.description.versionFINAL_PUBLISHED
dc.description.volume10
dc.identifier.doi10.1038/S41598-020-62205-6
dc.identifier.issn2045-2322
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/118389
dc.identifier.weblinkhttps://www.nature.com/articles/s41598-020-62205-6
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofScientific Reports
dc.relation.pages1-5
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titleRenal carcinoma CD105-/CD44-cells display stem-like properties in vitro and form aggressive tumors in vivo.
dc.typeJournalArticle
dspace.entity.typePublication