Artykuł w czasopiśmie
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Dostęp zamkniętyCharge-transfer interactions of Cr species with DNA
| dc.abstract.en | Interactions of Cr species with nucleic acids in living organisms depend strongly on Cr oxidation state and the environmental conditions. As the effects of these interactions range from benign to pre-mutagenic to carcinogenic, careful assessment of the hazard they pose to human health is necessary. We have investigated methods that would enable quantifying the DNA damage caused by Cr species under varying environmental conditions, including UV, O2, and redox potential, using simple instrumental techniques which could be in future combined into a field-deployable instrumentation. We have employed electrochemical quartz crystal nanogravimetry (EQCN), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) to evaluate the extent of DNA damage expressed in terms of guanine oxidation yield (η) and changes in specific characteristics provided by these techniques. The effects of the interactions of Cr species with DNA were analyzed using a model calf thymus DNA (ctDNA) film on a gold electrode (Au@ctDNA) in different media, including: (i) Cr(VI), (ii) Cr(VI) reduced at − 0.2 V, (iii) Cr(III) + UV radiation + O2, and Cr(III), obtaining the η values: 7.4 ± 1.4, 1.5 ± 0.4, 1.1 ± 0.31%, and 0%, respectively, thus quantifying the hazard posed. The EIS measurements have enabled utilizing the decrease in charge-transfer resistance (Rct) for ferri/ferrocyanide redox probe at an Au@ctDNA electrode to assess the oxidative ctDNA damage by Cr(VI) species. In this case, circular dichroism indicates an extensive damage to the ctDNA hydrogen bonding. On the other hand, Cr(III) species have not induced any damage to ctDNA, although the EQCN measurements show an electrostatic binding to DNA |
| dc.affiliation | Uniwersytet Warszawski |
| dc.contributor.author | Matysiak-Brynda, Edyta |
| dc.contributor.author | Nowicka, Anna |
| dc.contributor.author | Hepel, Maria |
| dc.date.accessioned | 2024-01-24T19:12:21Z |
| dc.date.available | 2024-01-24T19:12:21Z |
| dc.date.issued | 2017 |
| dc.description.finance | Nie dotyczy |
| dc.description.volume | 175 |
| dc.identifier.doi | 10.1016/J.JINORGBIO.2017.07.019 |
| dc.identifier.issn | 0162-0134 |
| dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/102951 |
| dc.language | eng |
| dc.pbn.affiliation | chemical sciences |
| dc.relation.ispartof | Journal of Inorganic Biochemistry |
| dc.relation.pages | 148-153 |
| dc.rights | ClosedAccess |
| dc.sciencecloud | nosend |
| dc.subject.en | Cr-induced DNA damage |
| dc.subject.en | ctDNA film testing Guanine oxidation |
| dc.subject.en | Quartz crystal nanogravimetry |
| dc.subject.en | DNA film impedance |
| dc.subject.en | Circular dichroism spectroscopy |
| dc.title | Charge-transfer interactions of Cr species with DNA |
| dc.type | JournalArticle |
| dspace.entity.type | Publication |