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Time-course microarray analysis reveals differences between rranscriptional changes in tomato leaves triggered by mild and severe variants of potato spindle tuber viroid.

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cris.lastimport.scopus2024-02-12T20:49:28Z
dc.abstract.enViroids are small non-capsidated non-coding RNA replicons that utilize host factors for efficient propagation and spread through the entire plant. They can incite specific disease symptoms in susceptible plants. To better understand viroid-plant interactions, we employed microarray analysis to observe the changes of gene expression in “Rutgers” tomato leaves in response to the mild (M) and severe (S23) variants of potato spindle tuber viroid (PSTVd). The changes were analyzed over a time course of viroid infection development: (i) the pre-symptomatic stage; (ii) early symptoms; (iii) full spectrum of symptoms and (iv) the so-called ‘recovery’ stage, when stem regrowth was observed in severely affected plants. Gene expression profiles differed depending on stage of infection and variant. In S23-infected plants, the expression of over 3000 genes was affected, while M-infected plants showed 3-fold fewer differentially expressed genes, only 20% of which were specific to the M variant. The differentially expressed genes included many genes related to stress; defense; hormone metabolism and signaling; photosynthesis and chloroplasts; cell wall; RNA regulation, processing and binding; protein metabolism and modification and others. The expression levels of several genes were confirmed by nCounter analysis.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorIwanicka-Nowicka, Roksana
dc.contributor.authorGóra-Sochacka, Anna
dc.contributor.authorWięsyk, Aneta
dc.contributor.authorFogtman, Anna
dc.contributor.authorZagórski-Ostoja, Włodzimierz
dc.date.accessioned2024-01-26T10:53:25Z
dc.date.available2024-01-26T10:53:25Z
dc.date.issued2018
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number5
dc.description.versionFINAL_AUTHOR
dc.description.volume10
dc.identifier.doi10.3390/V10050257
dc.identifier.issn1999-4915
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/123416
dc.identifier.weblinkhttp://www.mdpi.com/1999-4915/10/5/257
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofViruses
dc.relation.pages257
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enviroid
dc.subject.enpotato spindle tuber viroid (PSTVd)
dc.subject.enmicroarray
dc.subject.entranscriptome analysis
dc.subject.endifferentially expressed genes
dc.subject.ennCounter analysis
dc.titleTime-course microarray analysis reveals differences between rranscriptional changes in tomato leaves triggered by mild and severe variants of potato spindle tuber viroid.
dc.typeJournalArticle
dspace.entity.typePublication