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Promoter‐pervasive transcription causes <scp>RNA</scp> polymerase <scp>II</scp> pausing to boost <scp><i>DOG1</i></scp> expression in response to salt

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cris.lastimport.scopus2024-02-12T20:49:36Z
dc.abstract.enEukaryotic genomes are pervasively transcribed by RNA polymerase II. Yet, the molecular and biological implications of such a phenomenon are still largely puzzling. Here, we describe noncoding RNA transcription upstream of the Arabidopsis thaliana DOG1 gene, which governs salt stress responses and is a key regulator of seed dormancy. We find that expression of the DOG1 gene is induced by salt stress, thereby causing a delay in seed germination. We uncover extensive transcriptional activity on the promoter of the DOG1 gene, which produces a variety of lncRNAs. These lncRNAs, named PUPPIES, are co-directionally transcribed and extend into the DOG1 coding region. We show that PUPPIES RNAs respond to salt stress and boost DOG1 expression, resulting in delayed germination. This positive role of pervasive PUPPIES transcription on DOG1 gene expression is associated with augmented pausing of RNA polymerase II, slower transcription and higher transcriptional burst size. These findings highlight the positive role of upstream co-directional transcription in controlling transcriptional dynamics of downstream genes.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPlewczyński, Dariusz
dc.contributor.authorSwiezewski, Szymon
dc.contributor.authorMassana, Ferran
dc.contributor.authorYatusevich, Ruslan
dc.contributor.authorWrona, Magdalena
dc.contributor.authorSacharowski, Sebastian
dc.contributor.authorBokota, Grzegorz
dc.contributor.authorKrzyszton, Michal
dc.contributor.authorMajchrowska, Maria
dc.contributor.authorMontez, Miguel
dc.date.accessioned2024-01-25T18:09:02Z
dc.date.available2024-01-25T18:09:02Z
dc.date.issued2023
dc.description.financePublikacja bezkosztowa
dc.description.number5
dc.description.volume42
dc.identifier.doi10.15252/EMBJ.2022112443
dc.identifier.issn0261-4189
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/117233
dc.identifier.weblinkhttps://onlinelibrary.wiley.com/doi/pdf/10.15252/embj.2022112443
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofEMBO Journal
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titlePromoter‐pervasive transcription causes <scp>RNA</scp> polymerase <scp>II</scp> pausing to boost <scp><i>DOG1</i></scp> expression in response to salt
dc.typeJournalArticle
dspace.entity.typePublication