Artykuł w czasopiśmie
Brak miniatury
Licencja
CC-BYCC-BY - Uznanie autorstwa
 

Searching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry

Uproszczony widok
dc.abstract.enA fit for purpose analytical protocol was designed towards searching for low olecular weight seleno-compounds in sprouts. Complementary analytical techniques were used to collect information enabling the characterization of selenium speciation. Conceiving the overall characterization of the behavior of selenium, inductively plasma optical mass spectrometry (ICP-MS)was used to determine the total selenium content in entire sprouts as well as in selected extracts orchromatographic fractions. Then, high-performance liquid chromatography combined with ICP-MS (HPLC-ICP-MS) was used to evaluate the presence of inorganic and organic seleno-compounds, with the advantages of being very sensitive towards selenium, but limited by available selenium standard compounds. Finally, ultra-high performance liquid chromatography electrospray ionization triple quadrupole mass spectrometry (UHPLC-ESI-QqQ-MS/MS) and UHPLC-ESI-Orbitrap-MS/MS were used for the confirmation of the identity of selected compounds and identification of several unknown compounds of selenium in vegetable sprouts (sunflower, onion, radish), respectively. Cultivation of plants was designed to supplement sprouts with selenium by using solutions of selenium (IV) at the concentration of 10, 20, 40, and 60 mg/L. The applied methodology allowed to justify that vegetable sprouts metabolize inorganic selenium to a number of organic derivatives, such as seleno-methylselenocysteine (SeMetSeCys), selenomethionine (SeMet), 50-seleno-adenosine, 2,3-DHP-selenolanthionine, Se-S conjugate of cysteine-selenoglutathione, 2,3-DHP-selenocysteinecysteine, 2,3-DHP-selenocysteine-cysteinealanine, glutathione-2,3-DHP-selenocysteine, gamma-Glu- MetSeCys or glutamyl-glycinyl-N-2,3-DHP-selenocysteine.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorTomiak, Anna
dc.contributor.authorKościuczuk, Olga
dc.contributor.authorMichalska-Kacymirow, Magdalena
dc.contributor.authorBulska, Ewa
dc.contributor.authorKonopka, Anna
dc.contributor.authorKurek, Eliza
dc.date.accessioned2024-01-25T20:04:30Z
dc.date.available2024-01-25T20:04:30Z
dc.date.copyright2020-06-22
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number12
dc.description.versionFINAL_PUBLISHED
dc.description.volume25
dc.identifier.doi10.3390/MOLECULES25122870
dc.identifier.issn1420-3049
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/119115
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/25/12/2870/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMolecules
dc.relation.pages2870
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enselenium speciation
dc.subject.enbiotransformation of selenium in plants
dc.subject.enlow molecular weight seleno-compounds
dc.subject.enmass spectrometry
dc.titleSearching for Low Molecular Weight Seleno-Compounds in Sprouts by Mass Spectrometry
dc.typeJournalArticle
dspace.entity.typePublication