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High-Throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells

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cris.lastimport.scopus2024-02-12T20:38:25Z
dc.abstract.enRNA turnover is an essential part of the gene expression pathway, and there are several experimental approaches for its determination. High-throughput measurement of global RNA turnover rates can provide valuable information about conditions or proteins that impact gene expression. Here, we present a protocol for mitochondrial RNA turnover analysis which involves metabolic labeling of RNA coupled with quantitative high-throughput fluorescent microscopy. This approach gives an excellent opportunity to discover new factors involved in mitochondrial gene regulation when combined with loss-of-function screening strategy.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKotrys, Anna V.
dc.contributor.authorSzczęsny, Roman
dc.contributor.authorBorowski, Łukasz
dc.date.accessioned2024-01-28T20:40:44Z
dc.date.available2024-01-28T20:40:44Z
dc.date.issued2021
dc.description.financeNie dotyczy
dc.identifier.doi10.1007/978-1-0716-0834-0_11
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/153541
dc.identifier.weblinkhttp://link.springer.com/content/pdf/10.1007/978-1-0716-0834-0_11
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.publisher.ministerialHumana Press
dc.relation.bookMitochondrial Gene Expression: Methods and Protocols
dc.relation.pages133-146
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enMitochondrial RNA
dc.subject.enBromouridine (BrU)
dc.subject.enRNA turnover
dc.subject.enMetabolic labeling
dc.titleHigh-Throughput Measurement of Mitochondrial RNA Turnover in Human Cultured Cells
dc.typeMonographChapter
dspace.entity.typePublication