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MALDI Spectra Database for Rapid Discrimination and Subtyping of Mycobacterium kansasii.
dc.abstract.en | Mycobacterium kansasii is an emerging non-tuberculous mycobacterial (NTM) pathogen capable of causing severe lung disease. Of the seven currently recognized M. kansasii genotypes (I-VII), genotypes I and II are most prevalent and have been associated with human disease, whereas the other five (III-VII) genotypes are predominantly of environmental origin and are believed to be non-pathogenic. Subtyping of M. kansasii serves as a valuable tool to guide clinicians in pursuing diagnosis and to initiate the proper timely treatment. Most of the previous rapid diagnostic tests for mycobacteria employing the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology focused on species-level identification. The purpose of this study was to establish MALDI-TOF MS reference spectra database for discrimination of M. kansasii at the genotype level. A panel of 32 strains, representatives of M. kansasii genotypes I-VI were selected, whole cell proteins extracted and measured with MALDI-TOF MS. A unique main spectra (MSP) library was created using MALDI Biotyper Compass Explorer software. The spectra reproducibility was assessed by computing composite correlation index and MSPs cross-matching. One hundred clinical M. kansasii isolates used for testing of the database resulted in 90% identification at genus-level, 7% identification at species-level and 2% identification was below the threshold of log score value 1.7, of which all were correct at genotype level. One strain could not be identified. On the other hand, 37% of strains were identified at species level, 40% at genus level and 23% was not identified with the manufacturer's database. The MALDI-TOF MS was proven a rapid and robust tool to detect and differentiate between M. kansasii genotypes. It is concluded that MALDI-TOF MS has a potential to be incorporated into the routine diagnostic workflow of M. kansasii and possibly other NTM species. |
dc.affiliation | Uniwersytet Warszawski |
dc.contributor.author | Ingen, Jakko van |
dc.contributor.author | Bakuła, Zofia |
dc.contributor.author | Ulmann, Vit |
dc.contributor.author | Lewin, Astrid |
dc.contributor.author | Kamal, Elizabeth |
dc.contributor.author | Roesler, Uwe |
dc.contributor.author | Murugaiyan, Jayaseelan |
dc.contributor.author | Jagielski, Tomasz |
dc.contributor.author | Safianowska, Aleksandra |
dc.contributor.author | Barañano, Miren Unzaga |
dc.contributor.author | Humięcka, Joanna |
dc.date.accessioned | 2024-01-25T05:31:55Z |
dc.date.available | 2024-01-25T05:31:55Z |
dc.date.issued | 2018 |
dc.description.finance | Nie dotyczy |
dc.identifier.doi | 10.3389/FMICB.2018.00587 |
dc.identifier.issn | 1664-302X |
dc.identifier.uri | https://repozytorium.uw.edu.pl//handle/item/111737 |
dc.language | eng |
dc.pbn.affiliation | biological sciences |
dc.relation.ispartof | Frontiers in Microbiology |
dc.rights | ClosedAccess |
dc.sciencecloud | nosend |
dc.subject.en | Biotyper |
dc.subject.en | Mycobacterium kansasii |
dc.subject.en | genotypes |
dc.subject.en | matrix-assisted laser desorption ionization—time of flight mass spectrometry |
dc.subject.en | microflex |
dc.title | MALDI Spectra Database for Rapid Discrimination and Subtyping of Mycobacterium kansasii. |
dc.type | JournalArticle |
dspace.entity.type | Publication |