Artykuł w czasopiśmie
Brak miniatury
Licencja
CC-BYCC-BY - Uznanie autorstwa
 

Regulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid.

Uproszczony widok
cris.lastimport.scopus2024-02-12T20:47:24Z
dc.abstract.enPhosphatidic acid (PA) is involved in the regulation of plant growth and development, as well as responses to various environmental stimuli. Several PA targets in plant cells were identified, including two SNF1-related protein kinases 2 (SnRK2s), SnRK2.10 and SnRK2.4, which are not activated by abscisic acid (ABA). Here, we investigated the effects of PA on various elements of ABA-non-activated SnRK2 signaling. PA 16:0/18:1 was found to modulate the SnRK2 structure and the phosphorylation of some SnRK2 targets. Conversely, phosphorylation by the ABA-non-activated SnRK2s, of one of such targets, dehydrin Early Responsive to Dehydration 14 (ERD14), affects its interaction with PA and subcellular localization. Moreover, PA 16:0/18:1 modulates the activity and/or localization of negative regulators of the ABA-non-activated SnRK2s, not only of the ABA insensitive 1 (ABI1) phosphatase, which was identified earlier, but also of another protein phosphatase 2C, PP2CA. The activity of both phosphatases was inhibited by about 50% in the presence of 50 μM PA. PA 16:0/18:1 also impacts the phosphorylation and subcellular localization of SnRK2-interacting calcium sensor, known to inhibit SnRK2 activity in a calcium-dependent manner. Thus, PA was found to regulate ABA-non-activated SnRK2 signaling at several levels: the activity, phosphorylation status and/or localization of SnRK2 cellular partners.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorKlimecka, Maria
dc.contributor.authorBucholc, Maria
dc.contributor.authorKrzywińska, Ewa
dc.contributor.authorSzczegielniak, Jadwiga
dc.contributor.authorDobrowolska, Grażyna
dc.contributor.authorMaszkowska, Justyna
dc.contributor.authorLichocka, Małgorzata
dc.contributor.authorGoch, Grażyna
dc.date.accessioned2024-01-25T19:09:37Z
dc.date.available2024-01-25T19:09:37Z
dc.date.copyright2020-07-15
dc.date.issued2020
dc.description.accesstimeAT_PUBLICATION
dc.description.financePublikacja bezkosztowa
dc.description.number14
dc.description.versionFINAL_PUBLISHED
dc.description.volume21
dc.identifier.doi10.3390/IJMS21144984
dc.identifier.issn1422-0067
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/118227
dc.identifier.weblinkhttps://doi.org/10.3390/ijms21144984
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofInternational Journal of Molecular Sciences
dc.relation.pages4984 (1-21)
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enArabidopsis thaliana
dc.subject.enSnRK2-interacting calcium sensor
dc.subject.enSCS
dc.subject.endehydrins
dc.subject.enERD14
dc.subject.enPP2CA
dc.subject.enSnRK2
dc.subject.enSNF1-related protein kinases
dc.subject.enphosphatidic acid
dc.subject.enPA
dc.titleRegulation of ABA-Non-Activated SNF1-Related Protein Kinase 2 Signaling Pathways by Phosphatidic Acid.
dc.typeJournalArticle
dspace.entity.typePublication