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PCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera

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dc.abstract.enFungi of the Scopulariopsis genus, commonly found in the environment, are opportunistic pathogens that can cause various types of human infections. So far, no efficient molecular method has been developed for species differentiation among Scopulariopsis and related genera. In order to advance this field, we have evaluated performance of polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) assays, based on cytochrome c oxidase subunit 1 and β-tubulin genes. The assays resulted in 2–10 restriction patterns, depending on the gene amplified and restriction enzyme applied. Pooled analysis of the patterns allowed to propose an algorithm, that can be successfully used for an accurate species-specific identification of 21 species of the Scopulariopsis-like fungi.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBakuła, Zofia
dc.contributor.authorKordalewska, Milena
dc.contributor.authorKalita, Joanna
dc.contributor.authorBrillowska-Dąbrowska, Anna
dc.contributor.authorJagielski, Tomasz
dc.date.accessioned2024-01-25T16:22:24Z
dc.date.available2024-01-25T16:22:24Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number5
dc.description.volume57
dc.identifier.doi10.1093/MMY/MYY106
dc.identifier.issn1369-3786
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115631
dc.identifier.weblinkhttp://academic.oup.com/mmy/article-pdf/57/5/643/28683868/myy106.pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofMedical Mycology
dc.relation.pages643 - 648
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enIDENTIFICATION, OPPORTUNISTIC PATHOGENS, PCR, RFLP, SCOPULARIOPSIS
dc.titlePCR-RFLP assays for species-specific identification of fungi belonging to Scopulariopsis and related genera
dc.typeJournalArticle
dspace.entity.typePublication