Artykuł w czasopiśmie
Brak miniatury
Licencja
ClosedAccessDostęp zamknięty
 

Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins

Uproszczony widok
cris.lastimport.scopus2024-02-12T20:47:52Z
dc.abstract.enUtilization of non-AUG alternative translation start sites is most common in bacteria and viruses, but it has been also reported in other organisms. This phenomenon increases proteome complexity by allowing expression of multiple protein isoforms from a single gene. In Saccharomyces cerevisiae, a few described cases concern proteins that are translated from upstream near-cognate start codons as N-terminally extended variants that localize to mitochondria. Using bioinformatics tools, we provide compelling evidence that in yeast the potential for producing alternative protein isoforms by non-AUG translation initiation is much more prevalent than previously anticipated and may apply to as many as a few thousand proteins. Several hundreds of candidates are predicted to gain a mitochondrial targeting signal (MTS), generating an unrecognized pool of mitochondrial proteins. We confirmed mitochondrial localization of a subset of proteins previously not identified as mitochondrial, whose standard forms do not carry an MTS. Our data highlight the potential of non-canonical translation initiation in expanding the capacity of the mitochondrial proteome and possibly also other cellular features.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorWrobel, Lidia
dc.contributor.authorAlam, Jahangir
dc.contributor.authorNiemitalo, Olli
dc.contributor.authorMiścicka, Anna
dc.contributor.authorMonteuuis, Geoffray
dc.contributor.authorKastaniotis, Alexander J
dc.contributor.authorZenad, Lounis
dc.contributor.authorŚwirski, Michał
dc.contributor.authorKufel, Joanna
dc.contributor.authorChacińska, Agnieszka
dc.date.accessioned2024-01-25T13:50:41Z
dc.date.available2024-01-25T13:50:41Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number11
dc.description.volume47
dc.identifier.doi10.1093/NAR/GKZ301
dc.identifier.issn0305-1048
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/113825
dc.identifier.weblinkhttp://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gkz301/28509546/gkz301.pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofNucleic Acids Research
dc.relation.pages5777-5791
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleNon-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins
dc.typeJournalArticle
dspace.entity.typePublication