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Cyclosporine A, in Contrast to Rapamycin, Affects the Ability of Dendritic Cells to Induce Immune Tolerance Mechanisms

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dc.abstract.enFollowing organ transplantation, it is essential that immune tolerance is induced in the graft recipient to reduce the risk of rejection and avoid complications associated with the long-term use of immunosuppressive drugs. Immature dendritic cells (DCs) are considered to promote transplant tolerance and may minimize the risk of graft rejection. The aim of the study was to evaluate the effects of immunosuppressive agents: rapamycin (Rapa) and cyclosporine A (CsA) on generation of human tolerogenic DCs (tolDCs) and also to evaluate the ability of these cells to induce mechanisms of immune tolerance. tolDCs were generated in the environment of Rapa or CsA. Next, we evaluated the effects of these agents on surface phenotypes (CD11c, MHC II, CD40, CD80, CD83, CD86, CCR7, TLR2, TLR4), cytokine production (IL-4, IL-6, IL-10, IL-12p70, TGF-β), phagocytic capacity and resistant to lipopolysaccharide activation of these DCs. Moreover, we assessed ability of such tolDCs to induce T cell activation and apoptosis, Treg differentiation and production of Th1- and Th2-characteristic cytokine profile. Data obtained in this study demonstrate that rapamycin is effective at generating maturation-resistant tolDCs, however, does not change the ability of these cells to induce mechanisms of immune tolerance. In contrast, CsA affects the ability of these cells to induce mechanisms of immune tolerance, but is not efficient at generating maturation-resistant tolDCs.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorJankowska, Aleksandra
dc.contributor.authorKotur, Monika
dc.contributor.authorKotkowska, Zuzanna
dc.contributor.authorŁaski, Artur
dc.contributor.authorMaruszewska-Cheruiyot, Marta
dc.contributor.authorMachcińska, Maja
dc.contributor.authorKorczak-Kowalska, Grażyna
dc.contributor.authorBocian, Katarzyna
dc.date.accessioned2024-01-24T21:15:16Z
dc.date.available2024-01-24T21:15:16Z
dc.date.issued2021
dc.description.financeŚrodki finansowe, o których mowa w art. 365 pkt. 2 ustawy
dc.description.number1
dc.description.volume69
dc.identifier.doi10.1007/S00005-021-00632-7
dc.identifier.issn0004-069X
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/104075
dc.identifier.weblinkhttps://link.springer.com/content/pdf/10.1007/s00005-021-00632-7.pdf
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofArchivum Immunologiae et Therapiae Experimentalis
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.subject.enDendritic cells, Immune tolerance, T cells, Immunosuppressive agents, Rapamycin, Cyclosporine A
dc.titleCyclosporine A, in Contrast to Rapamycin, Affects the Ability of Dendritic Cells to Induce Immune Tolerance Mechanisms
dc.typeJournalArticle
dspace.entity.typePublication