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Reversible Freeze-Induced β-Sheet-to-Disorder Transition in Aggregated Homopolypeptide System

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cris.lastimport.scopus2024-02-12T20:48:52Z
dc.abstract.enConformational transitions involving aggregated proteins or peptides are of paramount biomedical and biotechnological importance. Here, we report an unusual freeze-induced structural reorganization within a !-sheet-rich ionic coaggregate of poly(L-lysine), PLL, and poly(L-glutamic acid), PLGA. Freezing aqueous suspensions of the PLL−PLGA !-aggregate in the presence of low concentrations of salt (NaBr) induces an instantaneous !-sheet-to-disorder transition, as probed by infrared spectroscopy in the amide I# band region. The conformational rearrangement of polypeptide chains appears to be fully synchronized with the global liquid-to-ice phase transition. In contrast to the known freeze-induced transitions, the process described here is fully reversible: the subsequent thawing results in an instantaneous disorder-to-!-sheet “refolding”. However, in the absence of traces of soluble salts, the !-sheet framework of the PLL−PLGA aggregate remains resistant to freezing as no transition is observed. We note that the occurrence of the transition depends on the type of salt present in the sample. Our results highlight a hidden dimension of the structural dynamics within !-sheet-rich aggregates. Possible scenarios of freeze-induced salt-bridge rupture and removal of water from nanocanals are discussed.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorFortunka, Mateusz
dc.contributor.authorŚcibisz, Grzegorz
dc.contributor.authorDec, Robert
dc.contributor.authorPuławski, Wojciech
dc.contributor.authorDzwolak, Wojciech
dc.contributor.authorGuza, Marcin
dc.contributor.authorWacławska, Matylda
dc.date.accessioned2024-01-25T19:25:57Z
dc.date.available2024-01-25T19:25:57Z
dc.date.issued2019
dc.description.financeNie dotyczy
dc.description.number43
dc.description.volume123
dc.identifier.doi10.1021/ACS.JPCB.9B06097
dc.identifier.issn1520-6106
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/118550
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofJournal of Physical Chemistry B
dc.relation.pages9080-9086
dc.rightsClosedAccess
dc.sciencecloudnosend
dc.titleReversible Freeze-Induced β-Sheet-to-Disorder Transition in Aggregated Homopolypeptide System
dc.typeJournalArticle
dspace.entity.typePublication