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Molecular dissection of the replication system of plasmid pIGRK encoding two in-frame Rep proteins with antagonistic functions

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dc.abstract.enBackground Gene overlapping is a frequent phenomenon in microbial genomes. Excluding so-called “trivial overlapping”, there are significant implications of such genetic arrangements, including regulation of gene expression and modification of protein activity. It is also postulated that, besides gene duplication, the appearance of overlapping genes (OGs) is one of the most important factors promoting a genome’s novelty and evolution. OGs coding for in-frame proteins with different functions are a particularly interesting case. In this study we identified and characterized two in-frame proteins encoded by OGs on plasmid pIGRK from Klebsiella pneumoniae, a representative of the newly distinguished pHW126 plasmid family. Results A single repR locus located within the replication system of plasmid pIGRK encodes, in the same frame, two functional polypeptides: a full-length RepR protein and a RepR’ protein (with N-terminal truncation) translated from an internal START codon. Both proteins form homodimers, and interact with diverse DNA regions within the plasmid replication origin and repR promoter operator. Interestingly, RepR and RepR’ have opposing functions – RepR is crucial for initiation of pIGRK replication, while RepR’ is a negative regulator of this process. Nevertheless, both proteins act cooperatively as negative transcriptional regulators of their own expression. Conclusions Regulation of the initiation of pIGRK replication is a complex process in which a major role is played by two in-frame proteins with antagonistic functions. In-frame encoded Rep proteins are uncommon, having been described in only a few plasmids. This is the first description of such proteins in a plasmid of the pHW126 family.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorBaran, Piotr
dc.contributor.authorWawrzyniak, Paweł
dc.contributor.authorGościk, Agata
dc.contributor.authorBierczyńska-Krzysik, Anna
dc.contributor.authorŁukasiewicz, Natalia
dc.contributor.authorKabaj, Paulina
dc.contributor.authorBartosik, Dariusz
dc.contributor.authorZaleski, Piotr
dc.contributor.authorMazurkiewicz-Pisarek, Anna
dc.contributor.authorSobolewska-Ruta, Agnieszka
dc.contributor.authorKierył, Piotr
dc.contributor.authorPłucienniczak, Andrzej
dc.date.accessioned2024-01-25T12:53:12Z
dc.date.available2024-01-25T12:53:12Z
dc.date.copyright2019-11-13
dc.date.issued2019
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.description.volume19
dc.identifier.doi10.1186/S12866-019-1595-3
dc.identifier.issn1471-2180
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/112857
dc.identifier.weblinkhttps://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-019-1595-3
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofBMC Microbiology
dc.relation.pages1-15 (article no. 254)
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enOverlapping genes
dc.subject.enPlasmid replication
dc.subject.enReplication system
dc.subject.enRep protein
dc.subject.enIn-frame proteins
dc.subject.enTranscription regulation
dc.subject.enKlebsiella pneumoniae
dc.titleMolecular dissection of the replication system of plasmid pIGRK encoding two in-frame Rep proteins with antagonistic functions
dc.typeJournalArticle
dspace.entity.typePublication