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Phase-variable Type I methyltransferase M.NgoAV from Neisseria gonorrhoeae FA1090 regulates phasevarion expression and gonococcal phenotype

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cris.lastimport.scopus2024-02-12T20:46:48Z
dc.abstract.enThe restriction-modification (RM) systems are compared to a primitive, innate, prokaryotic immune system, controlling the invasion by foreign DNA, composed of methyltransferase (MTase) and restriction endonuclease. The biological significance of RM systems extends beyond their defensive function, but the data on the regulatory role of Type I MTases are limited. We have previously characterized molecularly a non-canonical Type I RM system, NgoAV, with phase-variable specificity, encoded by Neisseria gonorrhoeae FA1090. In the current work, we have investigated the impact of methyltransferase NgoAV (M.NgoAV) activity on gonococcal phenotype and on epigenetic control of gene expression. For this purpose, we have constructed and studied genetic variants (concerning activity and specificity) within M.NgoAV locus. Deletion of M.NgoAV or switch of its specificity had an impact on phenotype of N. gonorrhoeae. Biofilm formation and planktonic growth, the resistance to antibiotics, which target bacterial peptidoglycan or other antimicrobials, and invasion of human epithelial host cells were affected. The expression of genes was deregulated in gonococcal cells with knockout M.NgoAV gene and the variant with new specificity. For the first time, the existence of a phasevarion (phase-variable regulon), directed by phase-variable Type I MTase, is demonstrated. Copyright © 2022 Adamczyk-Poplawska, Bacal, Mrozek, Matczynska, Piekarowicz and Kwiatek.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorAdamczyk-Popławska, Monika
dc.contributor.authorKwiatek, Agnieszka
dc.contributor.authorBącal, Paweł
dc.contributor.authorPiekarowicz, Andrzej
dc.contributor.authorMatczynska, Natalia
dc.contributor.authorMrozek, Agnieszka
dc.date.accessioned2024-01-25T16:35:49Z
dc.date.available2024-01-25T16:35:49Z
dc.date.issued2022
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.versionFINAL_PUBLISHED
dc.description.volume13
dc.identifier.doi10.3389/FMICB.2022.917639
dc.identifier.issn1664-302X
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/115784
dc.identifier.weblinkhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.917639/full
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofFrontiers in Microbiology
dc.rightsCC-BY
dc.sciencecloudnosend
dc.titlePhase-variable Type I methyltransferase M.NgoAV from Neisseria gonorrhoeae FA1090 regulates phasevarion expression and gonococcal phenotype
dc.typeJournalArticle
dspace.entity.typePublication