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Rindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production

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dc.abstract.enThe in vitro cultures of Rindera graeca, a rare endemic plant, were developed as a sustainable source of phenolic acids. Various shoot and root cultures were established and scaled up in a sprinkle bioreactor. A multiplication rate of 7.2 shoots per explant was achieved. HPLC–PDA–ESI–HRMS analysis revealed the presence of rosmarinic acid (RA) and lithospermic acid B (LAB) as the main secondary metabolites in both the shoot and root cultures. The maximum RA (30.0 ± 3.2 mg/g DW) and LAB (49.3 ± 15.5 mg/g DW) yields were determined in root-regenerated shoots. The strongest free radical scavenging activity (87.4 ± 1.1%), according to 2,2-diphenyl-1-picrylhydrazyl-hydrate assay, was noted for roots cultivated in a DCR medium. The highest reducing power (2.3 µM ± 0.4 TE/g DW), determined by the ferric-reducing antioxidant power assay, was noted for shoots cultivated on an SH medium containing 0.5 mg/L 6-benzylaminopurine. A genetic analysis performed using random amplified polymorphic DNA and start codon targeted markers revealed genetic variation of 62.8% to 96.5% among the investigated shoots and roots. This variability reflects the capacity of cultivated shoots and roots to produce phenolic compounds.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorPietrosiuk, Agnieszka
dc.contributor.authorZakrzewski, Patryk
dc.contributor.authorStępień, Piotr
dc.contributor.authorSzyszko, Ewa
dc.contributor.authorChinou, Ioanna
dc.contributor.authorGraikou, Konstantia
dc.contributor.authorJeziorek, Małgorzata
dc.contributor.authorKawka, Mateusz
dc.contributor.authorWileńska, Beata
dc.contributor.authorKuźma, Łukasz
dc.contributor.authorGaweł, Małgorzata
dc.contributor.authorSykłowska-Baranek, Katarzyna
dc.date.accessioned2024-01-25T19:26:47Z
dc.date.available2024-01-25T19:26:47Z
dc.date.copyright2023-06-20
dc.date.issued2023
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number12
dc.description.versionFINAL_PUBLISHED
dc.description.volume28
dc.identifier.doi10.3390/MOLECULES28124880
dc.identifier.issn1420-3049
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/118623
dc.identifier.weblinkhttps://www.mdpi.com/1420-3049/28/12/4880/pdf
dc.languageeng
dc.pbn.affiliationchemical sciences
dc.relation.ispartofMolecules
dc.relation.pages4880
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enantioxidants
dc.subject.enphenolic compounds
dc.subject.enmicropropagation
dc.subject.enhairy roots
dc.subject.enanatomical roots
dc.subject.enbioreactor
dc.subject.enRAPD
dc.subject.enSCoT
dc.titleRindera graeca (A. DC.) Boiss. & Heldr. (Boraginaceae) In Vitro Cultures Targeting Lithospermic Acid B and Rosmarinic Acid Production
dc.typeJournalArticle
dspace.entity.typePublication