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Stabilized nanosystem of nanocarriers with an immobilized biological factor for anti-tumor therapy

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dc.abstract.enObjective The inadequate efficiency of existing therapeutic anti-cancer regiments and the increase in the multidrug resistance of cancer cells underscore the need to investigate novel anticancer strategies. The induction of apoptosis in tumors by cytotoxic agents produced by pathogenic microorganisms is an example of such an approach. Nevertheless, even the most effective drug should be delivered directly to targeted sites to reduce any negative impact on other cells. Accordingly, the stabilized nanosystem (SNS) for active agent delivery to cancer cells was designed for further application in local anti-tumor therapy. A product of genetically modified Escherichia coli, listeriolysin O (LLO), was immobilized within the polyelectrolyte membrane (poly(ethylenimine)|hyaluronic acid) shells of ‘LLO nanocarriers’ coupled with the stabilizing element of natural origin. Methods and results The impact of LLO was evaluated in human leukemia cell lines in vitro. Correspondingly, the influence of the SNS and its elements was assessed in vitro. The viability of targeted cells was evaluated by flow cytometry. Visualization of the system structure was performed using confocal microscopy. The membrane shell applied to the nanocarriers was analyzed using atomic force microscopy and Fourier transform infrared spectroscopy techniques. Furthermore, the presence of a polyelectrolyte layer on the nanocarrier surface and/or in the cell was confirmed by flow cytometry. Finally, the structural integrity of the SNS and the corresponding release of the fluorescent solute listeriolysin were investigated. Conclusion The construction of a stabilized system offers LLO release with a lethal impact on model eukaryotic cells. The applied platform design may be recommended for local anti-tumor treatment purposes.
dc.affiliationUniwersytet Warszawski
dc.contributor.authorGranicka, Ludomira
dc.contributor.authorStrawski, Marcin
dc.contributor.authorGrubek, Zuzanna
dc.contributor.authorKamiński, Michał
dc.contributor.authorGRZECZKOWICZ, Anna
dc.contributor.authorStachowiak, Radosław
dc.contributor.authorSzklarczyk, Marek
dc.contributor.authorBielecki, Jacek
dc.contributor.authorKwiatkowska, Angelika
dc.date.accessioned2024-01-26T08:07:37Z
dc.date.available2024-01-26T08:07:37Z
dc.date.copyright2017-02-06
dc.date.issued2017
dc.description.accesstimeAT_PUBLICATION
dc.description.financeNie dotyczy
dc.description.number2
dc.description.versionFINAL_PUBLISHED
dc.description.volume12
dc.identifier.doi10.1371/JOURNAL.PONE.0170925
dc.identifier.issn1932-6203
dc.identifier.urihttps://repozytorium.uw.edu.pl//handle/item/120557
dc.identifier.weblinkhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0170925
dc.languageeng
dc.pbn.affiliationbiological sciences
dc.relation.ispartofPLoS ONE
dc.relation.pagese0170925
dc.rightsCC-BY
dc.sciencecloudnosend
dc.subject.enNanocarriers
dc.subject.enFlow cytometry
dc.subject.enBionanotechnology
dc.subject.enMicrobeads
dc.subject.enAtomic force microscopy
dc.subject.enCancer treatment
dc.subject.enCytostatics
dc.subject.enEukaryotic cells
dc.titleStabilized nanosystem of nanocarriers with an immobilized biological factor for anti-tumor therapy
dc.typeJournalArticle
dspace.entity.typePublication